Patent Alert

A U.S. Patent for "pH Adjustment to Improve Thaw Recovery of Cell Banks" was awarded to Genentech on March 31st, 2020. The present invention is a method of improving thaw recovery of cell banks comprising freezing CHO cells for banking in a freezing medium, wherein the freezing medium comprises a buffered solution and a cryoprotective agent, and wherein the freezing medium has a pH of 7.7 to 8.3 prior to freezing. Learn More

A U.S. Patent for "Materials and Methods for Extended Continuous Flow Fermentation of Reduced Genome Bacteria" was awarded to Scarab Genomics on March 31st, 2020. The present invention is a continuous fermentation process for the production of a biological product comprising culturing a population of reduced genome Escherichia coli (E. coli) bacteria cells comprising an inducible expression vector encoding a biological product in at least two successive fermentors, each configured as an independent continuous flow chemostat, said fermentors comprising a first fermentor comprising culture medium... Learn More

A U.S. Patent for "Host Cells for Use in an Inducible Coexpression System" was awarded to AbSci on March 31st, 2020. The present invention is a host cell suitable for expression of at least one gene product from two or more inducible promoters, wherein at least one of said inducible promoters is responsive to an inducer that is different than the inducer of another of said inducible promoters, and each inducible promoter is selected from the group consisting of: an L-arabinose-inducible promoter, a propionate-inducible promoter, a rhamnose-inducible promoter, and a D-xylose-inducible promoter; wherein the host cell has an altered gene function... Learn More

A U.S. Patent for "Isolating Cells Expressing Secreted Proteins" was awarded to Regeneron on March 24th, 2020. The present invention is a method of high expression of a protein of interest, comprising: (a) transfecting a mammalian cell with (i) a nucleic acid encoding the protein of interest and (ii) a nucleic acid encoding a cell surface capture molecule that comprises a membrane anchor and is capable of binding the protein of interest; (b) culturing the mammalian cell under conditions in which the protein of interest and cell surface capture molecule are expressed, and a protein of interest-cell surface capture molecule complex is formed and displayed on the cell surface of the mammalian cell... Learn More

A U.S. Patent Application for "Methods, Apparatuses, and Systems for Continuously Inactivating a Virus During Manufacture of a Biological Product " was published by Boehringer Ingelheim on March 19th, 2020. The present invention is an apparatus for continuously inactivating a virus during manufacture of a biological product, the apparatus comprising: an initial-mixing vessel; a pre-treatment detector chamber; a pre-treatment hold reservoir; a drain valve; and a treatment vessel that comprises an inlet, an outlet, and a static mixer, the inlet and the outlet being positioned at opposite ends of a major axis of the treatment vessel... Learn More

A U.S. Patent Application for "Methods for Increasing Mannose Content of Recombinant Proteins" was published by Amgen on March 19th, 2020. The present invention is a method for modulating mannose 5 on a recombinant protein during a mammalian cell culture process comprising: establishing a cell culture of mammalian cells in a cell culture medium, wherein the mammalian cells produce a recombinant protein; limiting the amount of glucose in the cell culture medium, wherein the concentration of the glucose is from about 0 to 6 g/L; and supplementing the cell culture medium with galactose or sucrose... Learn More

A U.S. Patent for "Recombinant Host Cell for Expressing Proteins of Interest" was awarded to Boehringer Ingelheim on March 17th, 2020. The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products. Learn More

A U.S. Patent for "Compositions for Cell Culture and Methods of Using the Same" was awarded to Bristol Myers Squibb on March 17th, 2020. The present invention is a cell culture process for the production of protein, comprising: a) culturing Chinese Hamster Ovary (CHO) cells which produce a protein of interest in cell culture under conditions that allow for protein production; and b) adding one or more antioxidant selected from the group consisting of catechin, chlorogenic acid, pelargonidin, quercetin, resveratrol, rosmarinic acid and silibinin to the cell culture, wherein protein titer is increased compared to the same cell culture without the antioxidant supplementation. Learn More

A U.S. Patent Application for "Mammalian Cell Cryopreservation Liquid" was published by Otsuka Pharma on March 12th, 2020. The present invention is a liquid for cryopreserving a cell and a liquid for administration of a mammalian cell capable of cryopreserving a mammalian cell and effectively suppressing cell death after thawing, and a method for cryopreserving a mammalian cell using the cell cryopreservation liquid. The liquid is an isotonic solution that includes 2.0 to 6.0% (w/v) of trehalose or a derivative thereof, or a salt of the trehalose or the derivative, 4.0 to 7.0% (w/v) of dextran or a derivative thereof... Learn More

A U.S. Patent Application for "Feed Media" was published by Amgen on March 12th, 2020. The present invention is a method for stabilizing a concentrated feed medium for feeding a mammalian cell culture comprising including in the feed medium at least about 9 mM pyruvate, wherein the feed medium comprises cysteine and/or cystine, wherein the sum of the concentrations of cysteine and/or cystine is at least about 7.9 mM, wherein the pH of the feed medium is from about 5.8 to about 7.4, wherein the feed medium comprises tyrosine and the tyrosine concentration is not more than about 4.4 mM, and wherein the feed medium with the included pyruvate is stable... Learn More

A U.S. Patent Application for "Methods for Cryogenic Storage" was published by Juno Therapeutics on March 12th, 2020. The present invention is a method comprising cryogenically storing cells from a biological sample derived from a donor, wherein the cells are obtained from the donor at a point in time that is after the donor is diagnosed with, or deemed to have or be suspected of having, a disease or condition, and before the donor has received one or more treatments for the disease or condition; and wherein the cells are frozen in a controlled rate freezer using a stepwise freezing profile comprising at least one step wherein the sample and/or chamber is cooled at a rate greater than 1.degree. C. per minute. Learn More

A U.S. Patent Application for "Microcarrier Perfusion Culturing Methods and Uses Thereof" was published by Genzymeon March 12th, 2020. The present invention is a method for testing a manufacturing process for making a recombinant protein, the method comprising: providing a shake tube containing an adherent Chinese hamster ovary (CHO) cell containing a nucleic acid encoding a recombinant protein disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 10% to about 30% of the volume of the shake tube and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L... Learn More

A U.S. Patent for "Process Control Systems and Methods for Use with Filters and Filtration Processes " was awarded to Amgenon March 10th, 2020. The present invention is a process control system comprising: (a) one or more filtration or purification processing units each operating at a flow rate, the one or more processing units comprising at least one of filtration or purification processing units; (b) a tank connected downstream from the one or more processing units; (c) a filter having an inlet, a permeate outlet, and a retentate outlet connected to the tank; (d) a feed pump having an inlet connected to the tank and an outlet connected to the inlet of the filter... Learn More

A U.S. Patent for a "In Vivo Production of Proteins" was awarded to Moderna on March 10th, 2020. The present invention is a method of expressing a protein in a mammalian subject, the method comprising administering a pharmaceutical composition comprising a plurality of lipid nanoparticles encapsulating a polynucleotide, wherein the lipid nanoparticle comprises a biodegradable cationic lipid, a neutral lipid, cholesterol, and a PEGylated lipid and the plurality of lipid nanoparticles has a mean particle size of between 80 nm to 160 nm, and wherein the polynucleotide comprises: (a) an open reading frame encoding the protein consisting of nucleotides selected... Learn More

A U.S. Patent for a "Cell Culture Process" was awarded to Momenta Pharma on March 10th, 2020. The present invention is a method of manufacturing a preparation of adalimumab, comprising: culturing a cell in a medium comprising 1.5 g/L arginine to 20 g/L arginine under conditions in which the cell expresses adalimumab; isolating the adalimumab, thereby producing a preparation of adalimumab; and formulating the preparation into a drug product if the preparation meets a target value of one or more C-terminal variants of adalimumab, wherein the C-terminal variants differ in amino acid sequence only by the presence or absence of a lysine... Learn More

A U.S. Patent Application for "End to End Cell Therapy Automation" was published by Lonza on March 5th, 2020. The present invention is a method for automated production of a genetically modified immune cell culture, the method performed by a cell engineering system, comprising: (a) activating an immune cell culture with an activation reagent to produce an activated immune cell culture in a first chamber of the cell engineering system; (b) transducing the activated immune cell culture, the transducing comprising: i. transferring via a first sterile, closed connection, the activated immune cell culture from the first chamber to an electroporation unit... Learn More

A U.S. Patent Application for "Sterile Chromatography Resin and Use Thereof in Manufacturing Processes" was published by Genzyme on March 5th, 2020. The present invention are methods of reducing bioburden of a chromatography resin that include exposing a container including a composition including (i) a chromatography resin and (ii) a liquid including at least on alcohol to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one alcohol are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Learn More

A U.S. Patent for "Capture Purification Processes for Proteins Expressed in a Non-Mammalian System" was awarded to Amgen on March 3rd, 2020. The present invention are methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided. Learn More

A U.S. Patent for "Methods of Perfusion Culturing Using a Shake Flask and Microcarriers" was awarded to Genzyme on March 3rd, 2020. The present invention is a method of optimizing a manufacturing process of producing a recombinant protein, the method comprising: providing a shake flask containing a mammalian cell disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 20% to about 30% of the volume of the shake flask and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L; incubating the shake flask for a period of time at about 32.degree. C. to about 39.degree. C.... Learn More

A U.S. Patent for "Compositions and Methods for Protein Glycosylation" was awarded to GSK on March 3rd, 2020. The present invention is oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein. Learn More

A U.S. Patent Application for "Growth Control of Eukaryotic Cells" was published by Roche on February 27th, 2020. The present invention is a system configured to receive a target cell density and a target time and for each of a plurality of time intervals; receive a measured cell density of the eukaryotic cells in the culture medium of the first tank; compute a predicted cell density at target time as a function of at least the measured cell density; compare the predicted cell density and the target cell density; adjusting the temperature of the culture medium in the first tank if the predicted cell density deviates from the target cell density by increasing the temperature by 0.1.degree... Learn More

A U.S. Patent Application for "Use of Raman Spectroscopy in Downstream Purification" was published by Regeneronon February 27th, 2020. The present invention are Raman spectroscopy methods and systems for characterizing or quantifying a protein purification intermediate and/or final concentrated pool during production or manufacture are provided. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify protein purification intermediates critical quality attributes during downstream processing (i.e., after harvest of the protein purification intermediate). For example, the disclosed in situ Raman spectroscopy methods and systems... Learn More

A U.S. Patent for "Protein Separations Using an Acrylamide Containing Filter" was awarded to MilliporeSigma on February 25th, 2020. The present invention is a method of improving the filtration capacity of a virus filter during purification of a protein of interest, comprising subjecting a sample comprising said protein of interest to a prefiltration step prior to passing through said virus filter, said prefiltration step comprising contacting said sample with a porous solid support selected from the group consisting of chromatographic resin, a membrane, a porous bead, a porous monolith, a winged fiber, a woven fabric and a non-woven fabric... Learn More

A U.S. Patent for "Rapid Screening of Monoclonal Antibodies" was awarded to Cellply on February 25th, 2020. The present invention is a method for screening an antibody for a desired response comprising the steps of: a) making available one or more inverted open microwells, wherein a microchannel having a cross sectional area of micrometer dimensions provides fluid to the one or more inverted open microwells, the microchannel having, at a first end, a fluid inlet, at a second end, a fluid outlet, wherein the fluid flows in a direction from the fluid inlet to the fluid outlet, wherein each of the one or more inverted open microwells... Learn More

A U.S. Patent for a "System and Method for Regulating Cell Culture Based Production of Biologics" was awarded to Lonza on February 25th, 2020. The present invention is a system for performing real-time analysis on a chromatography column comprising: a chromatography column; a conductivity meter; and at least one processor for executing stored program instructions to: measure one or more conductivity values of the chromatography column using the conductivity meter, determine a baseline conductivity value by recording a predetermined number of conductivity values from a first column volume to a second column volume and calculating an average... Learn More

A U.S. Patent for "Seed Train Processes and Uses Thereof" was awarded to Genzyme on February 25th, 2020. The present invention is a method of producing a recombinant protein, wherein the method consists of: (a) disposing a plurality of recombinant mammalian cells into a first culture medium within a vessel to provide a first cell culture; (b) batch culturing the first cell culture to a cell density range of about 1.0.times.10.sup.6 cells/mL to about 5.0.times.10.sup.6 cells/mL; (c) disposing a volume of the first cell culture of step (b) into a second culture medium within a perfusion bioreactor to provide a second cell culture with an initial cell density... Learn More

A U.S. Patent for a "Process for Preparing Recombinant Insulin Using Microfiltration" was awarded to Merck on February 18th, 2020. The present invention is a process for producing insulin or insulin analog, wherein the process has a digestion step in which an aqueous solution of properly refolded pre-proinsulin or pre-proinsulin analog is digested with one or more proteolytic enzymes to produce an aqueous solution of insulin or insulin analog, digestion byproducts, and host cell impurities and a downstream purification or chromatography step, in which the insulin or insulin analog is separated from the digestion byproducts, and host cell impurities... Learn More

A U.S. Patent for "Bioreactors with Multiple or Adjustable-Position Agitator Designs" was awarded to Genentech on February 18th, 2020. The present invention described herein are bioreactor support structures configured to be used with containers having different designs, and methods for making and using such bioreactors. The support structures described herein may include two or more agitator motors and control systems or an adjustable-position agitator motor, a removable spacer and/or lid, multiple configurations for ports and probes, and multiple exhaust filter heating blankets... Learn More

A U.S. Patent for "Cross-Scale Modeling of Bioreactor Cultures Using Raman Spectroscopy" was awarded to Biogen on February 18th, 2020. The present invention is a method of assessing a bioreactor culture, the method comprising: (i) obtaining a Raman spectrum of a manufacturing-scale bioreactor culture using a probe configured inline with the bioreactor; and (ii) determining a culture parameter of the manufacturing-scale bioreactor culture using a multivariate partial least squares (PLS) model that relates the Raman spectrum to the culture parameter, wherein the model was developed based on one or more test bioreactor cultures... Learn More

A U.S. Patent for "Methods for Quantifying Particulates in Cell Culture" was awarded to Celularity on February 18th, 2020. The present invention are methods for quantifying and/or detecting sub-visible particulates in cell cultures. The methods provided herein allow for the determination of the presence of non-cell sub-visible particulates in cell cultures, i.e., sub-visible particulates that are not intact cells, and quantification of such sub-visible particulates. The methods provided herein comprise a step of breaking down, e.g., lysing, cells in a cell culture. By breaking down the cells in the cell culture, non-cell sub-visible particulates can readily be detected... Learn More

A U.S. Patent for "Cell Culture Media" was awarded to Merck KGaA on February 18th, 2020. The present invention relates to dry cell culture media comprising amino acid components of certain particle size. Some dry powder cell culture media show poor dissolving properties and result in turbid solutions when they are dissolved in aqueous solutions. Using amino acid components of certain particle sizes significantly reduces that problem. Learn More

A U.S. Patent for an "Auto-Sampling System for Automatically Acquiring a Sample" was awarded to Waters on February 11th, 2020. The present invention is a sampling system comprising an external sampling assembly in fluidic communication with a process sample manager is provided herein. The sampling system can automatically acquire sample from one or more sources of sample to prepare sample for injection into a column or detector. The external sampling assembly has an external sampling valve connected to an external pump and is in fluidic communication with a process sample manager. The external sampling valve... Learn More

relates to monovalent influenza vaccine formulations and vaccination regimes for immunising against influenza disease, their use in medicine, in particular their use in augmenting immune responses to various antigens, and to methods of preparation. In particular, the invention relates to monovalent influenza immunogenic compositions comprising an influenza antigen or antigenic preparation thereof from an influenza virus strain being associated with a pandemic outbreak or having the potential to be associated with a pandemic outbreak... Learn More

A U.S. Patent for "Expanding Cells in a Bioreactor" wasawarded to Terumo BCT on February 11th, 2020. The present invention is a system for expanding cells, the system comprising: a bioreactor; at least one motor configured to connect to and rotate the bioreactor; at least one fluid circulation path fluidly associated with the bioreactor; at least one pump for circulating fluid through the at least one fluid circulation path and the bioreactor; a processor configured to execute processor executable instructions; and a memory storing processor executable instructions that when executed by the processor perform a method comprising... Learn More

A U.S. Patent for "Selection Marker for Cell Transfection and Protein Production" was awarded to Sanofi on February 11th, 2020. The present invention is within the field of industrial protein production. The invention provides a novel expression system using dihydroorotate dehydrogenase (DHODH) as a selection marker in combination with leflunomide or a metabolite thereof, notably for use in mammalian cell lines. Expression vectors encoding DHODH, cell lines comprising said vectors and methods of producing recombinant proteins are also provided. Learn More

A U.S. Patent for "pH Responsive Mucoadhesive Polymeric Encapsulated Microorganisms" was awarded to MIT on February 4th, 2020. The present invention is a method of encapsulating microorganisms, or components thereof, for targeted enteric delivery to an animal host have been developed. In particular, the encapsulation provides a prolonged survival, extended retention and a pH-sensitive release of the encapsulated microorganisms or antigenic components thereof at targeted sites within the gastrointestinal tract. The formulations are useful for diagnostic, therapeutic and prophylactic purposes and can alter a host's microbial composition associated with a condition or a disease state. Learn More

A U.S. Patent for "Primary Cell Gene Editing" was awarded to PACT Pharmaon February 4th, 2020. The present invention is a method of treating a cancer in a human patient comprising: a. modifying a patient-derived T cell by a nuclease-mediated introduction of a non-viral polynucleotide into the T cell, wherein the non-viral polynucleotide comprises: i. first and second homology arms homologous to first and second endogenous sequences of the cell; ii. a TCR gene sequence positioned between the first and second homology arms; iii. a first P2A-coding sequence positioned upstream of the TCR gene sequence... Learn More

A U.S. Patent for "Waveguide-Based Optical Scanning Systems" was awarded to LDIP on February 4th, 2020. The present invention is a scanning sensor system, methods of use and kits for detecting a biologically active analyte are provided. The scanning sensor system includes a light source, a detector, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, and at least one adapter configured to couple with the substrate and provide optical communication between the light source, the waveguides of the substrate, and the detector. Learn More

A U.S. Patent for "Methods for Increasing the Capacity of Flow-Through Processes" was awarded to MilliporeSigma on February 4th, 2020. The present invention is a process for separating target proteins from non-target proteins, comprising: a) obtaining a liquid sample containing target proteins and non-target proteins; b) increasing the concentration of the target proteins and non-target proteins in the sample by an ultrafiltration process to produce a concentrated feed for delivery to a chromatography device, the ultrafiltration process being a recirculating tangential flow filtration (TFF) process, a single-pass TFF (SPTFF) process, or a TFF process... Learn More

A U.S. Patent for a "Method of Cell Culture" was awarded to Pfizer on January 28th, 2020. The present invention is a method of cell culture comprising (i) providing cells in a cell culture medium to start a fed-batch cell culture process, and, (ii) maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenyl pyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3mM, wherein maintaining the at least one metabolite below a concentration C1 in the cell culture comprises the step... Learn More

A U.S. Patent for a "Disposable Liquid Chemical Sensor System" was awarded to Entegris on January 28th, 2020. The present invention is a disposable sensor system comprising: at least one disposable sensor assembly comprising a threaded cap with an index of refraction sensor bonded to the threaded cap, said index of refraction sensor measures a chemical concentration based on a refractive index, said sensor configured to perform data acquisition representing a chemical concentration, the disposable sensor assembly is affixed through a port assembly to a disposable polymeric bag container, the disposable polymeric bag comprising an optical window integrated... Learn More

A U.S. Patent for "Methods of Replicating a Large Scale Eculizumab Production Cell Culture" was awarded to Alexion on January 28th, 2020. The present invention is a method of replicating a large-scale eculizumab production cell culture in a small scale culture, the method comprising: adding 3.7 mL of a first lipid solution containing linoleic acid, oleic acid and cholesterol prior to inoculation; providing a 4-L to 10-L fed batch bioreactor containing a first culture medium that occupies between about 70% and about 90% of the capacity of the bioreactor and has an initial cell density of between about 2.5.times.10.sup.5 and about 7.5.times.10.sup.5 NS0 cells/mL... Learn More

A U.S. Patent for "Bioreactors and Uses Thereof" was awarded to Biostage on January 28th, 2020. The present invention is a bioreactor comprising: vessel having side walls defining a chamber, the side walls having a first end and a second end opposed to the first end, the chamber having at least one opening that communicates with the chamber, the at least one opening defined proximate to the first end of the side walls, the chamber being configured for containing a biological object under culture conditions; a cover component configured for covering the at least one opening located proximate to the first end of the side walls, the cover component removably connected to the vessel...
Learn More

A U.S. Patent for a "Method of Cell Culture" was awarded to Pfizer on January 28th, 2020. The present invention isa method of cell culture comprising (i) providing cells in a cell culture medium to start a fed-batch cell culture process, and, (ii) maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenyl pyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3mM, wherein maintaining the at least one metabolite below a concentration C1 in the cell culture comprises. Learn More

A U.S. Patent for "Tangential Flow Depth Filtration Systems and Methods of Filtration Using Same" was awarded to Repligen on January 21st, 2020. The present invention is a bioprocessing system comprising: a bioreactor; a tangential flow depth filtration (TFDF) unit comprising a melt-blown thick-walled hollow fiber formed from at least one polymer and comprising a porous wall having a pore size and a density, the porous wall defining a lumen that is in fluid communication with the bioreactor; a permeate fluid outlet in fluid communication with the porous wall; and a pump in fluid communication with the lumen; wherein the density is between 51% and 56% of the density of an equivalent solid volume of the polymer. Learn More

A U.S. Patent for a "Port and Related Container System" was awarded to Thermo Fisher on January 21st, 2020. The present invention is a container system that includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. The probe port also includes a flange coupled to the tubular member and radially outwardly projecting therefrom, the flange being secured to the flexible bag. Learn More

A U.S. Patent for a "Method for Improving the Stability of Purified Factor VIII After Reconstitution" was awarded to CSL on January 21st, 2020. The present invention relates to a method for increasing the stability of a Factor VIII molecule after purification, lyophilization and reconstitution, comprising preventing proteolytic cleavage of the Factor VIII molecule into a first fragment comprising essentially the A1 domain and the A2 domain and a second fragment comprising essentially the A3 domain, the C1 domain and the C2 domain throughout manufacturing of the Factor VIII molecule. Learn More

A U.S. Patent for a "Lyophilized Formulation of HGF" was awarded to Eisai on January 21st, 2020. The present invention provides a lyophilized formulation having improved storage stability of hepatic growth factor compared to conventional lyophilized formulations. The present invention provides a lyophilized formulation comprising (1) a hepatic growth factor, (2) trehalose and (3) one or more compounds selected from the group consisting of arginine, histidine, lysine, meglumine, glutamic acid, aspartic acid, proline, creatine, creatinine, tris(hydroxymethyl)aminomethane, and pharmaceutically acceptable salts thereof. Learn More

A U.S. Patent for "Methods and Compositions for Expression of Polypeptides in a Cell" was awarded to Celltheon on January 21st, 2020. The present invention are new methods for expression of polypeptides in cells that utilize new nucleic acid vectors, also disclosed herein. The vectors include transcriptional regulatory sequences derived from viral genomes, such as the cytomegalovirus (CMV) genome. In some embodiments, the expression vectors further include a post-transcriptional regulatory element (PRE). In some embodiments, the expression vectors further include a MAR/SAR sequence. These vectors provide higher expression levels of genes to which they are operatively linked... Learn More

A U.S. Patent for "Methods of Cell Culture for Adoptive Cell Therapy" was awarded to Baylor College on January 14th, 2020. The present invention is a static culture method for expanding a population of cells with a gas permeable cell culture device having a growth surface comprised of liquid impermeable gas permeable material, at least a portion of said gas permeable material in contact with an ambient gas, the method comprising: adding a population of desired cells and a population of antigen presenting and/or feeder cells and a medium into the gas permeable cell culture device, a ratio of the volume of said medium to a surface area of said growth... Learn More

A U.S. Patent for "Anti-Fouling Chiral Surfaces for Membrane Filtration and Methods Therefor" was awarded to the Rensselaer Polytechnic Institute on January 14th, 2020. The present invention relates to, inter alia, a modified surface comprising an optically active monomer, a polymeric material having a surface onto which the optically active monomer is covalently bound. In one aspect, a membrane comprising an optically active monomer, a poly(aryl sulfone) membrane having a surface onto which the optically active monomer is covalently bound. The present disclosure also relates to a method of modifying a surface... Learn More

A U.S. Patent for "Modular Parts that Supply Utilities to Cleanroom, Isolation or Containment Cubicles, Pods, or Modules" was awarded to G-Con Manufacturing on January 14th, 2020. The present invention is a removable portable utility unit for providing one or more utilities to at least one of a controlled air, a sealable, a sterilizable, a sanitizable portable cleanroom wherein the portable cleanroom is validatable for manufacturing pharmaceuticals or products that require high levels of cleanliness during manufacture, wherein the removable portable utility unit and the portable cleanroom comprises: a hermetic seal along a perimeter of a wall... Learn More

A U.S. Patent for a "Method, Device, and System for the Automated Determination of Optical Densities or of the Change in Optical Densities of Reaction Mixtures in Shaken Reactors" was awarded to Aquila Biolabs on January 14th, 2020. The present invention is a method for determining the optical density and/or the change in the optical density of a reaction mixture in a shaken reactor, comprising the following steps: providing light from at least one light source along a light path that enters the reaction mixture, detecting the light transmitted entirely through the reaction mixture and scattered from the reaction mixture in the light path by at least one light sensor... Learn More

A U.S. Patent for "Methods and Apparatus for Processing Fluids" was awarded to Life Technologies on January 7th, 2020. The present invention is a method for processing a fluid includes removably securing a retention member to a vessel that bounds a chamber; inserting a collapsible bag within the chamber of the vessel; securing the bag to the retention member so that the bag is supported within the chamber of the vessel; and dispensing a fluid into a compartment of the collapsible bag supported within the chamber of the vessel. The fluid can be mixed within bag while the bag is disposed within the vessel. Learn More

A U.S. Patent for a "Fed-Batch Process for the Production of Bacterial Ghosts" was awarded to Bird-C on January 7th, 2020. The present invention is a method for producing a bacterial ghost preparation comprising the steps: (a) providing bacterial cells comprising a lytic gene encoding a protein capable of forming a tunnel structure in the bacterial cell envelope wherein the expression of the lytic gene is under operative control of a regulatable promoter, (b) cultivating the bacterial cells under conditions wherein the lytic gene is not expressed to a final biomass concentration of about 10-80 g dry cell weight (DCW)/L, (c) after step (b)... Learn More

A U.S. Patent for "Stable IGG4 Based Binding Agent Formulations" was awarded to Sanofi on January 7th, 2020. The present invention provides stable pharmaceutical antibody formulations, including liquid drug product formulations and lyophilized drug product formulations, comprising an IgG4 binding agent and a citrate buffer, wherein the pH of the formulation is at or below both pH 6 and the pI of the binding agent. The formulations can be used in the treatment of chronic bowel diseases or rheumatoid arthritis. Learn More

A U.S. Patent for "Genetically Modified Mice that Produce Hybrid Antibodiese" was awarded to Regeneron on January 7th, 2020. The present invention is a method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method... Learn More

A U.S. Patent for the "Removal of Fragments from a Sample Containing a Target Protein Using Activated Carbon" was awarded to Merck KGaAon December 31st, 2019. The present invention is a method of reducing the amount of antibody fragments in a sample comprising an antibody to be purified, the method comprising the steps of: (a) providing a sample comprising an antibody and antibody fragments, wherein the fragments are in an amount equal to or greater than at least 0.2% of the amount of the antibody, (b) contacting the sample with activated carbon, wherein the activated carbon binds the antibody fragments... Learn More

A U.S. Patent for a "High-Throughput Cryogenic Spectroscopy for Glycan Analysis" was awarded to Ecole Polytechnique on December 31st, 2019. The present invention is a method for analyzing a plurality of molecules with cryogenic vibrational spectroscopy including the steps of providing a packet of molecules in a ionized form, injecting the packet into an ion mobility section, spatially separating the ions of the packet into subpackets according to their collisional cross section (CCS), recompressing the subpackets, by removing an empty space between them, loading the ions into a cryogenic ion trap... Learn More

A U.S. Patent for "Monoclonal Antibodies that Specifically Block Biological Activity of a Tumor Antigen" was awarded to Eisai on December 17th, 2019. The present invention relates to purified novel monoclonal antibodies that specifically bind to the alpha-folate receptor and compositions thereof. In some embodiments, the antibodies of the invention block the biological activity of FR-.alpha.. The antibodies and compositions of the invention are useful in the treatment of certain cancers, particularly cancers that have increased cell surface expression of the alpha-folate receptor... Learn More

A U.S. Patent for the "Recombinant Production of Mixtures of Antibodies" was awarded to Merus on December 17th, 2019. The present invention are methods for producing mixtures of antibodies from a single host cell clone, wherein, a nucleic acid sequence encoding a light chain and nucleic acid sequences encoding different heavy chains are expressed in a recombinant host cell. The recombinantly produced antibodies in the mixtures according to the invention suitably comprise identical light chains paired to different heavy chains capable of pairing to the light chain, thereby forming functional antigen-binding domains. Learn More

A U.S. Patent of "Methods for Purification of Antibodies Using Aliphatic Alcohols" was awarded to Novartis on December 10th, 2019. The present invention is a method for isolating a protein from a cell culture supernatant, the method comprising: a) combining a cell culture supernatant with a divalent cation salt under conditions suitable for the precipitation of impurities in the supernatant to produce a primary supernatant; b) combining the primary supernatant with an aliphatic alcohol under conditions suitable for forming a protein-containing precipitate and isolating the protein-containing precipitate; c) re-suspending the protein-containing precipitate in a buffer... Learn More

A U.S. Patent for a "Mixing and Filtering System" was awarded to Sani-Tech West on December 10th, 2019. The present invention is an apparatus for growing and separating cells or micro carriers from fluid medium in an aseptic environment, has a sealed, air and liquid tight, container having a port to allow for the introduction of fluid, gas or components, a filter with a filtration medium mounted centrally around an axis extending through the container, a mixing assembly that rotates around the filtration medium to allow for suspension of media or agitation thereof, and the mixing assembly has vanes containing a magnetic unit... Learn More

A U.S. Patent for "Protein Purification" was awarded to Genentech on December 10th, 2019. The present invention is a method for purifying an antibody from a composition comprising the antibody and a contaminant, which method comprises the following steps performed sequentially: (a) binding the antibody to a cation exchange material with an equilibration buffer at a first conductivity, wherein the amount of antibody in the composition loaded onto the cation exchange material is from about 15 mg to about 45 mg of the antibody per ml of cation exchange material; (b) washing the cation exchange material with a wash buffer with a conductivity... Learn More

A U.S. Patent for an "Apparatus and Method for Early Diagnosis of Cell Death" was awarded to Lyncee Tec on December 3rd, 2019. The present invention is an apparatus for monitoring cell viability in a sample, said apparatus comprising: an emitter for providing an electromagnetic wave for interaction with at least one cell of said sample; a measurement device configured to measure at least one optical property of said cell, said at least one optical property being probed with said electromagnetic wave and said at least one optical property being the phase of said electromagnetic wave interacting with said at least one cell... Learn More

A U.S. Patent for a "Bioreactor System" was awarded to GE on December 3rd, 2019. The present inventionis a method for regulating parameters of at least two bioreactor bags individually, which bioreactor bags are provided on one and the same rocking part of a bioreactor system, said method comprising the steps of: determining an individual weight of the content in each bioreactor bag at different points in time during processing; regulating one or more parameters in each bioreactor bag in dependence of the individual weights; wherein the step of determining an individual weight of the content in each bioreactor bag comprises... Learn More

A U.S. Patent Application for "Highly Multiplexed and Mass Spectrometry Based Methods to Measuring 72 Human Proteins" was published by Cedars-Sinai Medical Center
on December 5th, 2019. The present invention describes an automated trypsin digestion workflow that yields uniformly-processed samples in less than 5 hours. Reproducible quantitation of hundreds of peptides from numerous proteins was seen across replicates, days, instruments, and laboratory sites, demonstrating the broad applicability of this approach. Learn More

A U.S. Patent for "Etanercept Formulations Stabilized with Sodium Chloride" was awarded to Coherus on December 3rd, 2019. The present invention is an aqueous etanercept formulation stabilized to reduce instability, aggregation and/or fragmentation of the etanercept, said formulation comprising about 25 to about 75 mg/ml of etanercept and one or more stabilizers, wherein the stabilizers are selected from the group consisting of (i) sodium chloride; and (ii) sodium chloride in combination with sucrose or trehalose; and (iii) a combination of sodium chloride, sucrose and trehalose. Learn More

A U.S. Patent for the "In Vivo Production of Proteins" was awarded to Moderna Therapeutics on December 3rd, 2019. The present invention is a method of expressing a secreted protein in a mammalian subject, the method comprising administering a pharmaceutical composition comprising a plurality of lipid nanoparticles encapsulating a polynucleotide, wherein the lipid nanoparticle comprises a biodegradable cationic lipid, a neutral lipid, cholesterol, and a PEGylated lipid and the plurality of lipid nanoparticles has a mean particle size of between 80 nm to 160 nm, and wherein the polynucleotide comprises: (a) an open reading frame encoding the secreted protein... Learn More

A U.S. Patent for "Compositions Comprising Purified Anti-Abeta Monoclonal Antibodies" was awarded to Genentech on December 3rd, 2019. The present invention is a composition comprising an anti-Abeta monoclonal antibody purified from Chinese hamster ovary host cells, wherein the composition comprises the anti-Abeta antibody and a residual amount of hamster phospholipase B-domain-like 2 (PLBL2), wherein the amount of hamster PLBL2 is less than 20 ng/mg, wherein the anti-Abeta antibody comprises three heavy chain CDRs, CDR-H1 having the amino acid sequence of SEQ ID NO.:23, CDR-H2 having the amino acid sequence. Learn More

A U.S. Patent for "Data-Driven Prediction of Drug Combinations that Mitigate Adverse Drug Reactions" was awarded to IBM on November 26th, 2019. The present invention is a method for predicting beneficial drug combinations that mitigate adverse drug reactions, the method comprising: identifying a candidate set of drug combinations and target adverse drug reaction from at least one spontaneous reporting system comprising case reports of drugs and associated adverse drug reactions, each drug combination in the candidate set of drug combinations comprising a first drug associated with increasing a rate of occurrence... Learn More

A U.S. Patent Application for "Recombinant Yeast Strains for Pentose Fermentation" was published by Novozymes on November 21st, 2019. The present invention arerecombinant yeast cells expressing a xylulose kinase (XK) which are suitable for fermentation of pentoses. Also described are recombinant yeast cells with higher tolerance to formic and/or acetic acid and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing an enolase, a phosphofructokinase beta subunit, a 6-phosphofructo-2-kinase, a glucose-6-phosphate isomerase, a phosphoglycerate mutase and/or a triose-phosphate isomerase, and suitable for fermentation of pentoses. Learn More

A U.S. Patent Application for "Methods of Selecting Subjects for Combination Cancer Therapy with a Polymer-Conjugated Soluble" was published by Halozyme on November 21st, 2019. The present invention are methods of selecting subjects having cancer for combination therapy that includes administering an anti-hyaluronan agent, such as a polymer-conjugated hyaluronan-degrading enzyme. Combination therapies can include, in addition to the anti-hyaluronan agent, a tumor-targeted taxane and, optionally, a chemotherapeutic agent such as a nucleoside analog. Combinations for performing the methods are provided, as are uses of the combinations for selecting subjects for treatment. Learn More

A U.S. Patent for "Methods of Making T Cell Compositions" was awarded to Bluebird Bio on November 19th, 2019. The present invention is a method for manufacturing T cells comprising: a) activating a population of T cells and stimulating the population of T cells to proliferate; b) transducing the T cells with a viral vector comprising a polynucleotide encoding an engineered T cell receptor (TCR) or a chimeric antigen receptor (CAR); and c) culturing the transduced T cells to proliferate; wherein steps a)-c) are performed in the presence of a phosphatidylinositol-3 kinase (PI3K) inhibitor, and wherein T cell differentiation is decreased. Learn More

A U.S. Patent for "Method & Apparatus for Continuous Automated Perfusion System Harvesting from In-Situ Filtration Probe" was awarded to Flownamics on November 7th, 2019. The present invention is anapparatus and method for determining fluid flow (e.g., sterile media flow, filtrate flow, etc.) through tubing is provided. The apparatus includes an air source, a flow valve, a first sensor, a second sensor, and a media tubing section extending between the first sensor and the second sensor. In operation, air from the air source is introduced by the flow valve into a fluid flow to create a lead line of fluid immediately following the introduced air... Learn More

A U.S. Patent for "Method for Using Light Scattering in Real Time to Directly Monitor and Control Impurity Removal in Purification Processes" was awarded to Amgen on November 19th, 2019. The present invention is a methodfor controlling levels of biological impurities in an elution pool from a cation exchange chromatography resin comprising: loading a mixture containing a recombinant protein and biological impurities on to a cation exchange chromatography resin; eluting by a salt gradient the recombinant protein from the cation exchange chromatography resin; measuring the intensity of light scattered by the eluate with a laser light. Learn More

A U.S. Patent Application for "Epigenetic Analysis of Cell Therapy and Related Methods" was awarded to Juno Therapeutics on November 14th, 2019. The present invention is a method of identifying one or more genomic region(s) associated with an outcome of treatment with a cell therapy, the method comprising: (a) analyzing or determining an epigenetic property of one or more genomic regions of a cell or a population of cells, said cell or population comprised in (i) a first composition of cells to be genetically engineered with a recombinant receptor to produce a second composition comprising the recombinant receptor, or (ii) a second composition of cells. Learn More

A U.S. Patent Application for "Methods for Evaluating Monoclonality" was awarded to Lonza on November 14th, 2019. The present invention is a method of evaluating a value for probability of monoclonality, comprising: a) providing a solution comprising a population of cells; b) forming a plurality of aliquots of the solution; c) identifying aliquots having one cell; and d) providing, for aliquots identified as having one cell, a value for the probability that subsequent growth was monoclonal, thereby evaluating a value for probability of monoclonality. Learn More

A U.S. Patent Application for "Methods and Systems for T Cell Expansion" was awarded to Georgia Tech Research on November 14th, 2019. The present invention provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods of expanding, activating, and transfecting the suspension cells in the synthetic microenvironment, and suspension cells produced by such systems and methods. Learn More

A U.S. Patent for "Antigen-Binding Molecule Capable of Binding Two or More Antigen Molecules Repeatedly" was awarded to Chugai on November 12th, 2019. The present invention is a method of removing an antigen from plasma, the method comprising: administering an antibody to an individual in need of having an antigen removed from the individual's plasma, wherein the antibody binds to the antigen through the antigen-binding domain of the antibody and has a KD(pH5.8)/KD(pH7.4) value, defined as the ratio of KD for the antigen at pH 5.8 and KD for the antigen at pH 7.4, of 40 to 1,000, wherein the antibody binds to the antigen in plasma in vivo... Learn More

A U.S. Patent for "RNA Targeting Methods and Compositions" was awarded to the Salk Institute for Biological Studies on November 12th, 2019. The present invention is a method of targeting one or more target RNA molecules, comprising: contacting one or more target RNA molecules with a non-naturally occurring or engineered clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system comprising: at least one Cas13d protein comprising at least 95% sequence identity to SEQ ID NO: 189, or a nucleic acid molecule encoding the at least one Cas13d protein; and at least one CRISPR-Cas system guide RNA (gRNA)... Learn More

A U.S. Patent Application for "Self-Assembling Protein Nanostructures" was awarded to the University of Washington on November 7th, 2019. The present invention is a method, comprising: generating a plurality of representations of a first protein building block using a computing device; generating a plurality of representations of a second protein building block using the computing device, wherein the first protein building block differs from the second protein building block; generating an arrangement of the plurality of representations of the first protein building block and the plurality of representations of the second protein building block... Learn More

A U.S. Patent for "Microbial Production of Steviol Glycosides" was awarded to Manus Bio on November 5th, 2019. The present invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM. Learn More

A U.S. Patent for "Modified Polynucleotides for the Production of Cytoplasmic and Cytoskeletal Proteins" was awarded to Moderna on November 5th, 2019. The present invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility... Learn More

A U.S. Patent for a "Process and System for Obtaining Botulinum Neurotoxin" was awarded to Allergan on November 5th, 2019. The present invention is a process for producing a biologically active botulinum neurotoxin, the method comprising the following steps: providing a fermentation medium which is free of animal-derived products; fermenting Clostridium botulinum bacteria in the fermentation medium; contacting the fermentation medium with an anion exchange chromatography medium; then contacting an eluate of the anion exchange chrom medium with a cation exchange chromatography medium; and eluting an eluate. Learn More

A U.S. Patent Application for a "Method for Purifying Antibodies Using PBS" was published by the U.S. Department of Health and Human Services on October 31st, 2019. The present invention includes a method for purifying a biologic composition, comprising diafiltering the biologic composition into a composition comprising phosphate buffered saline (PBS) to obtain a purified composition. The method disclosed here can be particularly useful for removing one or more impurities from the biologic composition, such as bis methane (Bis-tris). Learn More

A U.S. Patent for "Preparing Antibodies from CHO Cell Cultures for Conjugation" was awarded to Seattle Genetics on October 29th, 2019. The present invention is based in part on the observation that a CHO cell oxidizing enzyme, particularly QSOX1, can survive a seemingly rigorous antibody purification process to reduce subsequent conjugation efficiency of the antibody to a drug. Whether the oxidizing enzyme survives the purification procedure depends on which purification techniques are employed which can vary from one antibody to another. Learn More

A U.S. Patent for "Efficient Selectivity of Recombinant Proteins" was  awarded to Regeneron  on October 29th, 2019 . The present invention provides a new expression system comprising a mammalian selectable marker that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems. Learn More

A U.S. Patent for "Robust Antibody Purification" was awarded to Merck on October 29th, 2019. The present invention is a method for the separation of host cell proteins, antibody fragments and low molecular weight substances from solutions containing antibodies, said method comprising: contacting a solution containing antibodies with a hydrophobic chromatography material for a period of time whereby the antibodies stay in solution and host cell proteins, antibody fragments and low molecular weight substances are adsorbed by the hydrophobic chromatography material... Learn More

A U.S. Patent Application for "Chromatography Column Qualification in Manufacturing Methods for Producing Anti-IL12/IL23 Antibody Compositions" was published by Janssen Biotech on October 24th, 2019. The present invention is a method of operating a chromatography column is described for use in methods of manufacture for producing anti-IL-12/IL-23p40 antibodies, e.g., the anti-IL-12/IL-23p40 antibody STELARA.RTM. (ustekinumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column... Learn More

A U.S. Patent for "Delivery Methods and Compositions for Nuclease-Mediated Genome Engineering" was awarded to Sangamo Therapeutics on October 22nd, 2019. The present invention is a method of introducing a donor nucleic acid molecule into an isolated cell, the method comprising: administering to the cell at least one adeno-associated virus (AAV) vector comprising a donor nucleic acid molecule in the presence of at least one inhibitor of Platelet-derived growth factor receptor under conditions sufficient to increase efficiency of viral delivery to the cell, wherein the AAV vector has an AAV6 capsid. Learn More

A U.S. Patent for a "Method of Using Human FC-Bearing IGG Antibodies to Polyethylene Glycol" was awarded to Bristol-Myers Squibb on October 22nd, 2019.  The present invention is a method of detecting human IgG anti-polyethylene glycol (PEG) antibodies in a sample from a human subject comprising: a. exposing a PEG-coated surface to a sample from a human subject, wherein the sample is blood, serum or any other blood fraction; b. washing the PEG-coated surface; c. detecting the presence or absence of human IgG anti-PEG antibodies bound to the PEG-coated surface; and d. determining the presence... Learn More

A U.S. Patent Application for "A Method of Enhancing the Performance of Chromatography Methods for Purification of Proteins" was published by A*STAR on October 17th, 2019. The present invention describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid... Learn More

A U.S. Patent Application for the "In Vivo Production of Proteins" was published by Moderna on October 17th, 2019. The present invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility to circulation... Learn More

A U.S. Patent for a "Fermentation Process for Producing Itaconic Acid Under Nitrogen Free Conditions" was awarded to DSM  on October 15th, 2019 . The present invention relates to a method for the production of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid in a suitable fermentation medium, thereby to produce itaconic acid, wherein: (a) the recombinant cell: (i) overexpresses cis-aconitate decarboxylase; and (ii) overexpresses a part of the citric acid cycle and/or has reduced activity of a native metabolic route to acetate and/or lactate; and, optionally, (b) the fermentation is carried out under anaerobic conditions. Learn More

A U.S. Patent for an "Impeller for Bioprocessing" was awarded to Pall  on October 15th, 2019 . The present invention is an impeller for a biocontainer comprising: (a) a hub including a top end and a bottom end, and at least first and second impeller arm slots arranged vertically and extending from the top end toward the bottom end; (b) at least first and second impeller blades extending from the hub, each impeller blade comprising a blade face, an arm having a first end and a second end and a notch between the first end and the second end, wherein the first end is adjacent to the blade face, and the second end extends into an impeller slot... Learn More

A U.S. Patent for a " Heat Transfer Baffle System and Uses Thereof"  was awarded to Abec on October 8th, 2019. The present invention is a reaction vessel comprising in its interior at least one heat transfer baffle, each heat transfer baffle comprising at least one subassembly comprising two distribution channels formed between a first material and a support material, at least one relief channel, and at least one closure bar having an adjustable width that determines the width of the relief channel and that is fixably attached to the support material, the support material further being attached to the reaction vessel at a substantially seamless boundary. Learn More

A U.S. Patent of "System and Method for Production of On-Demand Proteins in a Portable Unit for Point of Care Delivery" was awarded to the University of Maryland on October 8th, 2019.  The present invention is a method of delivering freshly synthesized therapeutic protein exhibiting increased activity and potency to a patient, said method comprising: delivering an on-demand cell-free synthesized therapeutic protein to a patient in need of said therapeutic protein, wherein said therapeutic protein is synthesized using a portable bioprocessing system comprising: a production module for producing the therapeutic protein, and a purification module. Learn More

A U.S. Patent on "Methods for Making Recombinant Proteins" was awarded to Coherus Biosciences on October 8th, 2019.  The present invention is a method of producing adalimumab in a mammalian cell culture comprising: a) inoculating a fed-batch bioreactor containing a cell culture production medium with mammalian cells comprising a nucleic acid encoding adalimumab or a fragment thereof wherein the mammalian cells are at a concentration from about 0.1 million to about 2 million cells/milliliter, wherein the production medium comprises glucose at an initial concentration from about 0.1 to about 0.9 grams/liter... Learn More

A U.S. Patent for "Rupture Disks for Bioreactors and Methods of Using Same" was awarded to Lonza on October 8th, 2019. The present invention is a rupture disk that includes a body including an internal wall extending from a first end of the body to a second end of the body. The internal wall defines an opening extending through the body. In some embodiments, the body further includes an internal flange formed on the internal wall, and the internal flange has a stepped surface. A rupture membrane is positioned within the body, and secured, for example, by cold welding. The rupture membrane may be secured to the stepped surface. Learn More