Patent Alert

A U.S. Patent for a "Lyophilized Formulation of HGF" was awarded to Eisai on January 21st, 2020. The present invention provides a lyophilized formulation having improved storage stability of hepatic growth factor compared to conventional lyophilized formulations. The present invention provides a lyophilized formulation comprising (1) a hepatic growth factor, (2) trehalose and (3) one or more compounds selected from the group consisting of arginine, histidine, lysine, meglumine, glutamic acid, aspartic acid, proline, creatine, creatinine, tris(hydroxymethyl)aminomethane, and pharmaceutically acceptable salts thereof. Learn More

A U.S. Patent for "Methods and Compositions for Expression of Polypeptides in a Cell" was awarded to Celltheon on January 21st, 2020. The present invention are new methods for expression of polypeptides in cells that utilize new nucleic acid vectors, also disclosed herein. The vectors include transcriptional regulatory sequences derived from viral genomes, such as the cytomegalovirus (CMV) genome. In some embodiments, the expression vectors further include a post-transcriptional regulatory element (PRE). In some embodiments, the expression vectors further include a MAR/SAR sequence. These vectors provide higher expression levels of genes to which they are operatively linked... Learn More

A U.S. Patent for "Methods of Cell Culture for Adoptive Cell Therapy" was awarded to Baylor College on January 14th, 2020. The present invention is a static culture method for expanding a population of cells with a gas permeable cell culture device having a growth surface comprised of liquid impermeable gas permeable material, at least a portion of said gas permeable material in contact with an ambient gas, the method comprising: adding a population of desired cells and a population of antigen presenting and/or feeder cells and a medium into the gas permeable cell culture device, a ratio of the volume of said medium to a surface area of said growth... Learn More

A U.S. Patent for "Anti-Fouling Chiral Surfaces for Membrane Filtration and Methods Therefor" was awarded to the Rensselaer Polytechnic Institute on January 14th, 2020. The present invention relates to, inter alia, a modified surface comprising an optically active monomer, a polymeric material having a surface onto which the optically active monomer is covalently bound. In one aspect, a membrane comprising an optically active monomer, a poly(aryl sulfone) membrane having a surface onto which the optically active monomer is covalently bound. The present disclosure also relates to a method of modifying a surface... Learn More

A U.S. Patent for "Modular Parts that Supply Utilities to Cleanroom, Isolation or Containment Cubicles, Pods, or Modules" was awarded to G-Con Manufacturing on January 14th, 2020. The present invention is a removable portable utility unit for providing one or more utilities to at least one of a controlled air, a sealable, a sterilizable, a sanitizable portable cleanroom wherein the portable cleanroom is validatable for manufacturing pharmaceuticals or products that require high levels of cleanliness during manufacture, wherein the removable portable utility unit and the portable cleanroom comprises: a hermetic seal along a perimeter of a wall... Learn More

A U.S. Patent for a "Method, Device, and System for the Automated Determination of Optical Densities or of the Change in Optical Densities of Reaction Mixtures in Shaken Reactors" was awarded to Aquila Biolabs on January 14th, 2020. The present invention is a method for determining the optical density and/or the change in the optical density of a reaction mixture in a shaken reactor, comprising the following steps: providing light from at least one light source along a light path that enters the reaction mixture, detecting the light transmitted entirely through the reaction mixture and scattered from the reaction mixture in the light path by at least one light sensor... Learn More

A U.S. Patent for "Methods and Apparatus for Processing Fluids" was awarded to Life Technologies on January 7th, 2020. The present invention is a method for processing a fluid includes removably securing a retention member to a vessel that bounds a chamber; inserting a collapsible bag within the chamber of the vessel; securing the bag to the retention member so that the bag is supported within the chamber of the vessel; and dispensing a fluid into a compartment of the collapsible bag supported within the chamber of the vessel. The fluid can be mixed within bag while the bag is disposed within the vessel. Learn More

A U.S. Patent for a "Fed-Batch Process for the Production of Bacterial Ghosts" was awarded to Bird-C on January 7th, 2020. The present invention is a method for producing a bacterial ghost preparation comprising the steps: (a) providing bacterial cells comprising a lytic gene encoding a protein capable of forming a tunnel structure in the bacterial cell envelope wherein the expression of the lytic gene is under operative control of a regulatable promoter, (b) cultivating the bacterial cells under conditions wherein the lytic gene is not expressed to a final biomass concentration of about 10-80 g dry cell weight (DCW)/L, (c) after step (b)... Learn More

A U.S. Patent for "Stable IGG4 Based Binding Agent Formulations" was awarded to Sanofi on January 7th, 2020. The present invention provides stable pharmaceutical antibody formulations, including liquid drug product formulations and lyophilized drug product formulations, comprising an IgG4 binding agent and a citrate buffer, wherein the pH of the formulation is at or below both pH 6 and the pI of the binding agent. The formulations can be used in the treatment of chronic bowel diseases or rheumatoid arthritis. Learn More

A U.S. Patent for "Genetically Modified Mice that Produce Hybrid Antibodiese" was awarded to Regeneron on January 7th, 2020. The present invention is a method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method... Learn More

A U.S. Patent for the "Removal of Fragments from a Sample Containing a Target Protein Using Activated Carbon" was awarded to Merck KGaAon December 31st, 2019. The present invention is a method of reducing the amount of antibody fragments in a sample comprising an antibody to be purified, the method comprising the steps of: (a) providing a sample comprising an antibody and antibody fragments, wherein the fragments are in an amount equal to or greater than at least 0.2% of the amount of the antibody, (b) contacting the sample with activated carbon, wherein the activated carbon binds the antibody fragments... Learn More

A U.S. Patent for a "High-Throughput Cryogenic Spectroscopy for Glycan Analysis" was awarded to Ecole Polytechnique on December 31st, 2019. The present invention is a method for analyzing a plurality of molecules with cryogenic vibrational spectroscopy including the steps of providing a packet of molecules in a ionized form, injecting the packet into an ion mobility section, spatially separating the ions of the packet into subpackets according to their collisional cross section (CCS), recompressing the subpackets, by removing an empty space between them, loading the ions into a cryogenic ion trap... Learn More

A U.S. Patent for "Monoclonal Antibodies that Specifically Block Biological Activity of a Tumor Antigen" was awarded to Eisai on December 17th, 2019. The present invention relates to purified novel monoclonal antibodies that specifically bind to the alpha-folate receptor and compositions thereof. In some embodiments, the antibodies of the invention block the biological activity of FR-.alpha.. The antibodies and compositions of the invention are useful in the treatment of certain cancers, particularly cancers that have increased cell surface expression of the alpha-folate receptor... Learn More

A U.S. Patent for the "Recombinant Production of Mixtures of Antibodies" was awarded to Merus on December 17th, 2019. The present invention are methods for producing mixtures of antibodies from a single host cell clone, wherein, a nucleic acid sequence encoding a light chain and nucleic acid sequences encoding different heavy chains are expressed in a recombinant host cell. The recombinantly produced antibodies in the mixtures according to the invention suitably comprise identical light chains paired to different heavy chains capable of pairing to the light chain, thereby forming functional antigen-binding domains. Learn More

A U.S. Patent of "Methods for Purification of Antibodies Using Aliphatic Alcohols" was awarded to Novartis on December 10th, 2019. The present invention is a method for isolating a protein from a cell culture supernatant, the method comprising: a) combining a cell culture supernatant with a divalent cation salt under conditions suitable for the precipitation of impurities in the supernatant to produce a primary supernatant; b) combining the primary supernatant with an aliphatic alcohol under conditions suitable for forming a protein-containing precipitate and isolating the protein-containing precipitate; c) re-suspending the protein-containing precipitate in a buffer... Learn More

A U.S. Patent for a "Mixing and Filtering System" was awarded to Sani-Tech West on December 10th, 2019. The present invention is an apparatus for growing and separating cells or micro carriers from fluid medium in an aseptic environment, has a sealed, air and liquid tight, container having a port to allow for the introduction of fluid, gas or components, a filter with a filtration medium mounted centrally around an axis extending through the container, a mixing assembly that rotates around the filtration medium to allow for suspension of media or agitation thereof, and the mixing assembly has vanes containing a magnetic unit... Learn More

A U.S. Patent for "Protein Purification" was awarded to Genentech on December 10th, 2019. The present invention is a method for purifying an antibody from a composition comprising the antibody and a contaminant, which method comprises the following steps performed sequentially: (a) binding the antibody to a cation exchange material with an equilibration buffer at a first conductivity, wherein the amount of antibody in the composition loaded onto the cation exchange material is from about 15 mg to about 45 mg of the antibody per ml of cation exchange material; (b) washing the cation exchange material with a wash buffer with a conductivity... Learn More

A U.S. Patent for an "Apparatus and Method for Early Diagnosis of Cell Death" was awarded to Lyncee Tec on December 3rd, 2019. The present invention is an apparatus for monitoring cell viability in a sample, said apparatus comprising: an emitter for providing an electromagnetic wave for interaction with at least one cell of said sample; a measurement device configured to measure at least one optical property of said cell, said at least one optical property being probed with said electromagnetic wave and said at least one optical property being the phase of said electromagnetic wave interacting with said at least one cell... Learn More

A U.S. Patent for a "Bioreactor System" was awarded to GE on December 3rd, 2019. The present inventionis a method for regulating parameters of at least two bioreactor bags individually, which bioreactor bags are provided on one and the same rocking part of a bioreactor system, said method comprising the steps of: determining an individual weight of the content in each bioreactor bag at different points in time during processing; regulating one or more parameters in each bioreactor bag in dependence of the individual weights; wherein the step of determining an individual weight of the content in each bioreactor bag comprises... Learn More

A U.S. Patent Application for "Highly Multiplexed and Mass Spectrometry Based Methods to Measuring 72 Human Proteins" was published by Cedars-Sinai Medical Center
on December 5th, 2019. The present invention describes an automated trypsin digestion workflow that yields uniformly-processed samples in less than 5 hours. Reproducible quantitation of hundreds of peptides from numerous proteins was seen across replicates, days, instruments, and laboratory sites, demonstrating the broad applicability of this approach. Learn More

A U.S. Patent for "Etanercept Formulations Stabilized with Sodium Chloride" was awarded to Coherus on December 3rd, 2019. The present invention is an aqueous etanercept formulation stabilized to reduce instability, aggregation and/or fragmentation of the etanercept, said formulation comprising about 25 to about 75 mg/ml of etanercept and one or more stabilizers, wherein the stabilizers are selected from the group consisting of (i) sodium chloride; and (ii) sodium chloride in combination with sucrose or trehalose; and (iii) a combination of sodium chloride, sucrose and trehalose. Learn More

A U.S. Patent for the "In Vivo Production of Proteins" was awarded to Moderna Therapeutics on December 3rd, 2019. The present invention is a method of expressing a secreted protein in a mammalian subject, the method comprising administering a pharmaceutical composition comprising a plurality of lipid nanoparticles encapsulating a polynucleotide, wherein the lipid nanoparticle comprises a biodegradable cationic lipid, a neutral lipid, cholesterol, and a PEGylated lipid and the plurality of lipid nanoparticles has a mean particle size of between 80 nm to 160 nm, and wherein the polynucleotide comprises: (a) an open reading frame encoding the secreted protein... Learn More

A U.S. Patent for "Compositions Comprising Purified Anti-Abeta Monoclonal Antibodies" was awarded to Genentech on December 3rd, 2019. The present invention is a composition comprising an anti-Abeta monoclonal antibody purified from Chinese hamster ovary host cells, wherein the composition comprises the anti-Abeta antibody and a residual amount of hamster phospholipase B-domain-like 2 (PLBL2), wherein the amount of hamster PLBL2 is less than 20 ng/mg, wherein the anti-Abeta antibody comprises three heavy chain CDRs, CDR-H1 having the amino acid sequence of SEQ ID NO.:23, CDR-H2 having the amino acid sequence. Learn More

A U.S. Patent for "Data-Driven Prediction of Drug Combinations that Mitigate Adverse Drug Reactions" was awarded to IBM on November 26th, 2019. The present invention is a method for predicting beneficial drug combinations that mitigate adverse drug reactions, the method comprising: identifying a candidate set of drug combinations and target adverse drug reaction from at least one spontaneous reporting system comprising case reports of drugs and associated adverse drug reactions, each drug combination in the candidate set of drug combinations comprising a first drug associated with increasing a rate of occurrence... Learn More

A U.S. Patent Application for "Recombinant Yeast Strains for Pentose Fermentation" was published by Novozymes on November 21st, 2019. The present invention arerecombinant yeast cells expressing a xylulose kinase (XK) which are suitable for fermentation of pentoses. Also described are recombinant yeast cells with higher tolerance to formic and/or acetic acid and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing an enolase, a phosphofructokinase beta subunit, a 6-phosphofructo-2-kinase, a glucose-6-phosphate isomerase, a phosphoglycerate mutase and/or a triose-phosphate isomerase, and suitable for fermentation of pentoses. Learn More

A U.S. Patent Application for "Methods of Selecting Subjects for Combination Cancer Therapy with a Polymer-Conjugated Soluble" was published by Halozyme on November 21st, 2019. The present invention are methods of selecting subjects having cancer for combination therapy that includes administering an anti-hyaluronan agent, such as a polymer-conjugated hyaluronan-degrading enzyme. Combination therapies can include, in addition to the anti-hyaluronan agent, a tumor-targeted taxane and, optionally, a chemotherapeutic agent such as a nucleoside analog. Combinations for performing the methods are provided, as are uses of the combinations for selecting subjects for treatment. Learn More

A U.S. Patent for "Methods of Making T Cell Compositions" was awarded to Bluebird Bio on November 19th, 2019. The present invention is a method for manufacturing T cells comprising: a) activating a population of T cells and stimulating the population of T cells to proliferate; b) transducing the T cells with a viral vector comprising a polynucleotide encoding an engineered T cell receptor (TCR) or a chimeric antigen receptor (CAR); and c) culturing the transduced T cells to proliferate; wherein steps a)-c) are performed in the presence of a phosphatidylinositol-3 kinase (PI3K) inhibitor, and wherein T cell differentiation is decreased. Learn More

A U.S. Patent for "Method & Apparatus for Continuous Automated Perfusion System Harvesting from In-Situ Filtration Probe" was awarded to Flownamics on November 7th, 2019. The present invention is anapparatus and method for determining fluid flow (e.g., sterile media flow, filtrate flow, etc.) through tubing is provided. The apparatus includes an air source, a flow valve, a first sensor, a second sensor, and a media tubing section extending between the first sensor and the second sensor. In operation, air from the air source is introduced by the flow valve into a fluid flow to create a lead line of fluid immediately following the introduced air... Learn More

A U.S. Patent for "Method for Using Light Scattering in Real Time to Directly Monitor and Control Impurity Removal in Purification Processes" was awarded to Amgen on November 19th, 2019. The present invention is a methodfor controlling levels of biological impurities in an elution pool from a cation exchange chromatography resin comprising: loading a mixture containing a recombinant protein and biological impurities on to a cation exchange chromatography resin; eluting by a salt gradient the recombinant protein from the cation exchange chromatography resin; measuring the intensity of light scattered by the eluate with a laser light. Learn More

A U.S. Patent Application for "Epigenetic Analysis of Cell Therapy and Related Methods" was awarded to Juno Therapeutics on November 14th, 2019. The present invention is a method of identifying one or more genomic region(s) associated with an outcome of treatment with a cell therapy, the method comprising: (a) analyzing or determining an epigenetic property of one or more genomic regions of a cell or a population of cells, said cell or population comprised in (i) a first composition of cells to be genetically engineered with a recombinant receptor to produce a second composition comprising the recombinant receptor, or (ii) a second composition of cells. Learn More

A U.S. Patent Application for "Methods for Evaluating Monoclonality" was awarded to Lonza on November 14th, 2019. The present invention is a method of evaluating a value for probability of monoclonality, comprising: a) providing a solution comprising a population of cells; b) forming a plurality of aliquots of the solution; c) identifying aliquots having one cell; and d) providing, for aliquots identified as having one cell, a value for the probability that subsequent growth was monoclonal, thereby evaluating a value for probability of monoclonality. Learn More

A U.S. Patent Application for "Methods and Systems for T Cell Expansion" was awarded to Georgia Tech Research on November 14th, 2019. The present invention provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods of expanding, activating, and transfecting the suspension cells in the synthetic microenvironment, and suspension cells produced by such systems and methods. Learn More

A U.S. Patent for "Antigen-Binding Molecule Capable of Binding Two or More Antigen Molecules Repeatedly" was awarded to Chugai on November 12th, 2019. The present invention is a method of removing an antigen from plasma, the method comprising: administering an antibody to an individual in need of having an antigen removed from the individual's plasma, wherein the antibody binds to the antigen through the antigen-binding domain of the antibody and has a KD(pH5.8)/KD(pH7.4) value, defined as the ratio of KD for the antigen at pH 5.8 and KD for the antigen at pH 7.4, of 40 to 1,000, wherein the antibody binds to the antigen in plasma in vivo... Learn More

A U.S. Patent for "RNA Targeting Methods and Compositions" was awarded to the Salk Institute for Biological Studies on November 12th, 2019. The present invention is a method of targeting one or more target RNA molecules, comprising: contacting one or more target RNA molecules with a non-naturally occurring or engineered clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system comprising: at least one Cas13d protein comprising at least 95% sequence identity to SEQ ID NO: 189, or a nucleic acid molecule encoding the at least one Cas13d protein; and at least one CRISPR-Cas system guide RNA (gRNA)... Learn More

A U.S. Patent Application for "Self-Assembling Protein Nanostructures" was awarded to the University of Washington on November 7th, 2019. The present invention is a method, comprising: generating a plurality of representations of a first protein building block using a computing device; generating a plurality of representations of a second protein building block using the computing device, wherein the first protein building block differs from the second protein building block; generating an arrangement of the plurality of representations of the first protein building block and the plurality of representations of the second protein building block... Learn More

A U.S. Patent for "Microbial Production of Steviol Glycosides" was awarded to Manus Bio on November 5th, 2019. The present invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM. Learn More

A U.S. Patent for "Modified Polynucleotides for the Production of Cytoplasmic and Cytoskeletal Proteins" was awarded to Moderna on November 5th, 2019. The present invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility... Learn More

A U.S. Patent for a "Process and System for Obtaining Botulinum Neurotoxin" was awarded to Allergan on November 5th, 2019. The present invention is a process for producing a biologically active botulinum neurotoxin, the method comprising the following steps: providing a fermentation medium which is free of animal-derived products; fermenting Clostridium botulinum bacteria in the fermentation medium; contacting the fermentation medium with an anion exchange chromatography medium; then contacting an eluate of the anion exchange chrom medium with a cation exchange chromatography medium; and eluting an eluate. Learn More

A U.S. Patent Application for a "Method for Purifying Antibodies Using PBS" was published by the U.S. Department of Health and Human Services on October 31st, 2019. The present invention includes a method for purifying a biologic composition, comprising diafiltering the biologic composition into a composition comprising phosphate buffered saline (PBS) to obtain a purified composition. The method disclosed here can be particularly useful for removing one or more impurities from the biologic composition, such as bis methane (Bis-tris). Learn More

A U.S. Patent for "Preparing Antibodies from CHO Cell Cultures for Conjugation" was awarded to Seattle Genetics on October 29th, 2019. The present invention is based in part on the observation that a CHO cell oxidizing enzyme, particularly QSOX1, can survive a seemingly rigorous antibody purification process to reduce subsequent conjugation efficiency of the antibody to a drug. Whether the oxidizing enzyme survives the purification procedure depends on which purification techniques are employed which can vary from one antibody to another. Learn More

A U.S. Patent for "Efficient Selectivity of Recombinant Proteins" was  awarded to Regeneron  on October 29th, 2019 . The present invention provides a new expression system comprising a mammalian selectable marker that promotes desirable post-translational modifications of glycoproteins. In particular, the invention includes methods and compositions for optimal recombinant protein expression in mammalian cells by employing a selection marker system based on GPT genes of mammalian origin. The invention includes methods that facilitate selectivity and enhanced expression copies as well as protein yield of recombinant proteins in mammalian cells, and methods of using GPT expression systems. Learn More

A U.S. Patent for "Robust Antibody Purification" was awarded to Merck on October 29th, 2019. The present invention is a method for the separation of host cell proteins, antibody fragments and low molecular weight substances from solutions containing antibodies, said method comprising: contacting a solution containing antibodies with a hydrophobic chromatography material for a period of time whereby the antibodies stay in solution and host cell proteins, antibody fragments and low molecular weight substances are adsorbed by the hydrophobic chromatography material... Learn More

A U.S. Patent Application for "Chromatography Column Qualification in Manufacturing Methods for Producing Anti-IL12/IL23 Antibody Compositions" was published by Janssen Biotech on October 24th, 2019. The present invention is a method of operating a chromatography column is described for use in methods of manufacture for producing anti-IL-12/IL-23p40 antibodies, e.g., the anti-IL-12/IL-23p40 antibody STELARA.RTM. (ustekinumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column... Learn More

A U.S. Patent for "Delivery Methods and Compositions for Nuclease-Mediated Genome Engineering" was awarded to Sangamo Therapeutics on October 22nd, 2019. The present invention is a method of introducing a donor nucleic acid molecule into an isolated cell, the method comprising: administering to the cell at least one adeno-associated virus (AAV) vector comprising a donor nucleic acid molecule in the presence of at least one inhibitor of Platelet-derived growth factor receptor under conditions sufficient to increase efficiency of viral delivery to the cell, wherein the AAV vector has an AAV6 capsid. Learn More

A U.S. Patent for a "Method of Using Human FC-Bearing IGG Antibodies to Polyethylene Glycol" was awarded to Bristol-Myers Squibb on October 22nd, 2019.  The present invention is a method of detecting human IgG anti-polyethylene glycol (PEG) antibodies in a sample from a human subject comprising: a. exposing a PEG-coated surface to a sample from a human subject, wherein the sample is blood, serum or any other blood fraction; b. washing the PEG-coated surface; c. detecting the presence or absence of human IgG anti-PEG antibodies bound to the PEG-coated surface; and d. determining the presence... Learn More

A U.S. Patent Application for "A Method of Enhancing the Performance of Chromatography Methods for Purification of Proteins" was published by A*STAR on October 17th, 2019. The present invention describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid... Learn More

A U.S. Patent Application for the "In Vivo Production of Proteins" was published by Moderna on October 17th, 2019. The present invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility to circulation... Learn More

A U.S. Patent for a "Fermentation Process for Producing Itaconic Acid Under Nitrogen Free Conditions" was awarded to DSM  on October 15th, 2019 . The present invention relates to a method for the production of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid in a suitable fermentation medium, thereby to produce itaconic acid, wherein: (a) the recombinant cell: (i) overexpresses cis-aconitate decarboxylase; and (ii) overexpresses a part of the citric acid cycle and/or has reduced activity of a native metabolic route to acetate and/or lactate; and, optionally, (b) the fermentation is carried out under anaerobic conditions. Learn More

A U.S. Patent for an "Impeller for Bioprocessing" was awarded to Pall  on October 15th, 2019 . The present invention is an impeller for a biocontainer comprising: (a) a hub including a top end and a bottom end, and at least first and second impeller arm slots arranged vertically and extending from the top end toward the bottom end; (b) at least first and second impeller blades extending from the hub, each impeller blade comprising a blade face, an arm having a first end and a second end and a notch between the first end and the second end, wherein the first end is adjacent to the blade face, and the second end extends into an impeller slot... Learn More

A U.S. Patent for a " Heat Transfer Baffle System and Uses Thereof"  was awarded to Abec on October 8th, 2019. The present invention is a reaction vessel comprising in its interior at least one heat transfer baffle, each heat transfer baffle comprising at least one subassembly comprising two distribution channels formed between a first material and a support material, at least one relief channel, and at least one closure bar having an adjustable width that determines the width of the relief channel and that is fixably attached to the support material, the support material further being attached to the reaction vessel at a substantially seamless boundary. Learn More

A U.S. Patent of "System and Method for Production of On-Demand Proteins in a Portable Unit for Point of Care Delivery" was awarded to the University of Maryland on October 8th, 2019.  The present invention is a method of delivering freshly synthesized therapeutic protein exhibiting increased activity and potency to a patient, said method comprising: delivering an on-demand cell-free synthesized therapeutic protein to a patient in need of said therapeutic protein, wherein said therapeutic protein is synthesized using a portable bioprocessing system comprising: a production module for producing the therapeutic protein, and a purification module. Learn More

A U.S. Patent on "Methods for Making Recombinant Proteins" was awarded to Coherus Biosciences on October 8th, 2019.  The present invention is a method of producing adalimumab in a mammalian cell culture comprising: a) inoculating a fed-batch bioreactor containing a cell culture production medium with mammalian cells comprising a nucleic acid encoding adalimumab or a fragment thereof wherein the mammalian cells are at a concentration from about 0.1 million to about 2 million cells/milliliter, wherein the production medium comprises glucose at an initial concentration from about 0.1 to about 0.9 grams/liter... Learn More

A U.S. Patent for "Rupture Disks for Bioreactors and Methods of Using Same" was awarded to Lonza on October 8th, 2019. The present invention is a rupture disk that includes a body including an internal wall extending from a first end of the body to a second end of the body. The internal wall defines an opening extending through the body. In some embodiments, the body further includes an internal flange formed on the internal wall, and the internal flange has a stepped surface. A rupture membrane is positioned within the body, and secured, for example, by cold welding. The rupture membrane may be secured to the stepped surface. Learn More

A U.S. Patent Application for "Perfusion Filtration Systems" was published by Artemis Biosystems on October 3rd, 2019. The present invention is a method of filtering a cell culture media comprising passing a cell culture media comprising mammalian cells at a viable cell density of at least 10.times.10.sup.6 cells per mL and a virus through a plurality of tubular porous membranes of a filter elements enclosed within a casing, wherein each tubular porous membrane filter element comprises a plurality of tubular porous membranes, wherein at least 95% of the pores in each of the plurality of tubular porous membranes are between 5 .mu.m and 15 .mu.m in size. Learn More

A U.S. Patent Application for "Control of Cell Growth Through a Temperature Feedback Loop" was published by Bristol-Myers Squibb on October 3rd, 2019. The present invention relates to the use of online biomass capacitance monitoring in cultures as a way to control the growth of cells through the use of a temperature control loop. In certain embodiments, a biomass capacitance probe is used to measure the cell density, and a predetermined growth curve is used to adjust the temperature in the culture. Learn More

A U.S. Patent for "Methods for Transduction and Cell Processing" was awarded to Juno Therapeutics on October 1st, 2019. The present invention is a method, system, and kit for cell processing, e.g., for therapeutic use, such as for adoptive cell therapy. The provided methods include transduction methods, in which cells and virus are incubated under conditions that result in transduction of the cells with a viral vector. The incubation in some embodiments is carried out in an internal cavity of a generally rigid centrifugal chamber, such as a cylindrical chamber made of hard plastic, the cavity of which may have a variable volume. Learn More

A U.S. Patent for a "Mixing Device for Mixing the Contents of a Container" was awarded to Sartorius on October 1st, 2019. The present invention is a mixing device for mixing contents of a container has a support for supporting the container and a moving device connected to the support for moving the support and mixing the contents of the container. The support has a positioning device to position the container so that a removal side of the container is arranged substantially along a predetermined line of the support. The support is pivotable about a removal pivot axis to move the container into a removal position. Learn More

A U.S. Patent for "Automated Multi-Step Purification System" was awarded to GE on October 1st, 2019. The present invention is an automated two step chromatography purification system comprising a, system controller, a capture flow path comprising at least one pump, an elution flow path comprising at least one pump, and a valve arrangement for selective connection of two capture columns to the capture flow path and the elution flow path respectively such that both flow paths may be operated simultaneously and in parallel. Learn More

A U.S. Patent Application for "Packaging for a Flexible Container and Transport Unit" was published by Sartorius Stedim Biotech on September 26th, 2019. The present invention is packaging for a flexible container, in particular for a disposable bag for use in a bioreactor, comprises a bottom part for fixing a first portion of the container, a ceiling part for fixing an opposite second portion of the container, a receiving space which on a first side is defined by the bottom part and on an opposite second side is defined by the ceiling part, and struts arranged between the bottom part and the ceiling part, which fix a defined distance between the bottom part and the ceiling part. Learn More

A U.S. Patent Application for and"Airlift Perfusion Bioreactor for the Culture of Cells" was published by the Keck Graduate Institute on September 26th, 2019. The present invention is a bioreactor apparatus, comprising: a cell culture zone comprising a riser section and a downcomer section; a settling zone comprising an inclined gravity settler in fluid communication with the downcomer section, the inclined gravity settler being partitioned from the riser section; a gas injection inlet to the riser section; a liquid inlet to the cell culture zone; and a liquid outlet on the inclined gravity settler. Learn More

A U.S. Patent for "Method and System for Leak Testing" was awarded to Pall on September 24th, 2019. The present invention is a method of leak testing a flexible expandable bag having at least one port and at least one flexible wall in a system comprising: a gas flow meter; a gas pressure regulator; a gas pressure transducer; a gas pressure relief valve; a displacement measurement sensor, arranged to measure displacement of the flexible wall of the bag as the bag expands while being filled with gas; at least three valves, each valve being controllable to allow or prevent gas flow therethrough. Learn More

A U.S. Patent for "Microcarrier Perfusion Culturing Methods and Uses Thereof" was published by Genzyme on September 24th, 2019. The present invention is a method of culturing an adherent Chinese hamster ovary (CHO) cell, the method comprising: providing a conical shake tube containing an adherent CHO cell disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 10% to about 30% of the volume of the conical shake tube and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L, wherein the microcarriers have (1) an average diameter of about 200 Tm to about 300 Tm. Learn More

A U.S. Patent for a "Method for the Clarification of High-Density Crude Cell Culture Harvest" was published by Janssen on September 24th, 2019. The present invention is a method for clarifying a crude cell culture harvest comprising cells as well as a secreted desired protein, wherein the cells are at a cell density at harvest of at least 15.times.10.sup.6 cells/ml and wherein at least 20% of said cells are viable, said method comprising the steps of: a. culturing cells in a bioreactor to a cell density of at least 15.times.10.sup.6 cells/ml in order to obtain a crude cell culture harvest comprising desired secreted proteins; b. subsequently processing at least 70% of the crude cell culture harvest obtained... Learn More

A U.S. Patent Application for "Culture Media for Culturing Pluripotent Stem Cells in Suspension" was published by Accellta on September 19th, 2019. The present invention is defined culture media for culturing pluripotent stem cells in a suspension culture devoid of substrate adherence, the defined culture media comprising an effective amount of a protease inhibitor; a GSK3.beta. inhibitor and at least one agent selected from the group consisting of a protease inhibitor and a WNT3A polypeptide; a WNT3A polypeptide and a stabilizing agent thereof with the proviso that said stabilizing agent is not a lipid vesicle; and/or a GSK3.beta. inhibitor, with the proviso that the medium is devoid of an ERK1/2 inhibitor. LEARN MORE

A U.S. Patent Application for "Methods for Improving Cell Viability In a Production Bioreactor" was published by Genzyme on September 19th, 2019. The present invention is a method of predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor, the method comprising: (a) determining cell viability under a plurality of test conditions in a test culture vessel, wherein the plurality of test conditions are defined by a set of parameters; and (b) based on the cell viability in the test culture vessel under the plurality of conditions, predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor. Learn More

A U.S. Patent Application for "Seeding an Adherent Cell Bioreactor with Non-Adherent Cells Increases Seeding Density Limit and Reduces Required Expansion Time" was published by Trizell on September 12th, 2019. The present invention is a counter-intuitive way to improve the commercial-scale production of recombinant biological products in adherent-cell bioreactors, which reduces the risk of cell culture contamination, increases total yield and reduces the delay between seeding and harvest, thus minimizing expression product degradation, by inter alia inoculating an adherent culture bioreactor with suspension-adapted producer cells. Learn More

A U.S. Patent Application for the "Detection of pH Utilizing Raman" was published by UK Research & Innovation on September 12th, 2019. The present invention includes methods and apparatus for measuring pH in a sub-surface volume of a diffusely scattering sample. Probe light is directed to an entry region on the sample surface, and collected from a collection region on the sample surface following diffuse scattering within the sample. The collection region is spatially offset from the entry region, so that when one or more Raman spectral features are detected in the collected probe light, a pH of the sub-surface volume can be determined from the spectral features. Learn More

A U.S. Patent Application for "High Efficiency Continuous Countercurrent Tangential Negative Chromatography" was published by Chromatan on September 12th, 2019. The present invention is a system, module and method for continuous or batch single-pass countercurrent tangential chromatography for bind/elute and negative chromatography applications. The system includes binding, washing, elution (for bind/elute), regeneration, and equilibration single-pass modules. The resin slurry flows in a continuous single pass at steady-state through each module, while corresponding buffers flow countercurrent to the slurry facilitating efficient product and impurity extraction. Learn More

A U.S. Patent Application for a "Chromatography Medium" was published by Puridify on September 12th, 2019. The present invention is a chromatography medium comprising one or more electrospun polymer nanofibres wherein the electrospun cellulose nanofibers are fused together at points where the nanofibers intersect one another, and which in use form a stationary phase comprising a plurality of pores through which a mobile phase can permeate, and wherein the chromatography medium has affinity chromatography protein ligand surface functionality interactive with target molecules. Learn More

A U.S. Patent for a "Particle Sensor with Interferent Discrimination" was awarded to BugLab on September 10th, 2019. The present invention provides methods and devices to measure particle suspension concentrations in the presence of potential interferents. Particle back-scatter readings are taken at light wavelengths that are absorbed by the medium before interacting with surrounding objects. Source-detector spacings are minimized compared to the mean absorbance path length of light, thereby maximizing the range of sensitivity to particle concentration. The active discrimination against potentially interfering particles, such as bubbles, is provided. Learn More

A U.S. Patent for a "Reagent Usable for the Isolation and/or Purification of Nucleic Acids" was awarded to Qiagen on September 10th, 2019. The present invention provides a special reagent for the pretreatment of sample materials. The pretreatment with the reagent according to the invention enables the isolation of nucleic acids by a uniform method from very diverse sample materials, in particular different bioprocess samples, even if the nucleic acids are only present in small amounts in the sample materials. The reagent used for the pretreatment comprises, as key component, at least one compound comprising an amino group. Learn More

A U.S. Patent Application for a "Signal Polypeptide for Improved Secretion of Protein" was published by MedImmune on September 5th, 2019. The present invention relates to a signal polypeptide for improving excretory production of a heterologous polypeptide, proteins comprising the signal polypeptide, nucleic acids encoding the signal polypeptide, and methods of producing thereof. Exemplary embodiments include an improved signal polypeptide attached to alpha toxin, and methods to produce and isolate alpha toxin. Learn More

A U.S. Patent Application for "Multivariate Spectral Analysis and Monitoring for Biomanufacturing" was published by Genzyme on September 5th, 2019. The present invention features methods that include obtaining a vibrational spectrum of a solution in a biological manufacturing system, analyzing the vibrational spectrum using a first chemometrics model to determine a value of a first quality attribute associated with the solution, analyzing the vibrational spectrum using a second chemometrics model to determine a value of a second quality attribute associated with the solution, and adjusting at least one parameter of a purification unit. Learn More

A U.S. Patent Application for "Chromatography Column Packing Medium Recovery" was published by Repligen on September 5th, 2019. The present invention includes a chromatography column tube having an inlet and outlet and port assembly arranged in a wall of the column tube between flow distributors that together form a chamber within the tube that is filled with packing medium are described. The port assembly facilitates the removal of resin from the pre-packed column and the port assembly does not affect fluid flow in the normal use of the column for chromatographic separation or ability to maintain sanitary conditions within the column. Learn More

A U.S. Patent for "Cell-Sorting Method and Associated Device" was awarded to Commissariat a L'Energie Atomique on September 3rd, 2019. The present invention is a method of discriminating a living cell from a dead cell, and a device for implementing the method, the method including: using a lens-free imaging device to acquire a diffraction figure corresponding to a cell; and determining a light intensity on a central area of an elementary diffraction figure associated with the cell. It can thus be determined if the studied cell is a living cell or a dead cell. Learn More

A U.S. Patent for "Compact Spiral-Wound Filter Elements, Modules and Systems" was awarded to MilliporeSigma on September 3rd, 2019. The present invention is a spiral-wound filter element comprising: a) a feed channel having a height of less than about 450 .mu.m and a length that is less than about 800 times the feed channel height, the feed channel including a mesh fiber feed screen; b) a permeate channel having a height of less than about 300 .mu.m, the permeate channel including a mesh fiber permeate screen; and c) a filtration membrane having a pore size in the range of about 1 nm to about 100 nm. Learn More

A U.S. Patent for "Sequencing Methods" was awarded to 10K Genomics on August 27th, 2019. The present invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention functions to improve the worth and significance of sequencing data. Learn More

A U.S. Patent for a "Method of Viral Purification" was awarded to GlaxoSmithKline  on August 27th, 2019. The present invention is a method of producing a virus in cell culture comprising at least the steps of: a) providing a population of cells in a cell culture medium, b) infecting the population of cells by: i. inoculating the population of cells with the virus, and ii. incubating the inoculated population of cells so as to allow the virus to replicate and propagate, c) collecting the produced virus, thereby providing a viral harvest, and d) purifying the virus, wherein a volumetric power input of at least 15... Learn More

A U.S. Patent Application for a "Process for Improving the Dissolution Behavior of Components in Aqueous Solutions" was published by MilliporeSigma on August 22nd, 2019. The present invention relates to a process for improving the solubility or dissolution behaviour of components in an aqueous solution. The dissolution properties of a slowly/badly dissolving component can be positively affected by generating mixed particles of this component with another component which has a negative dissolution enthalpy. By preparing and adding such mixed particles solubility is improved without changing the chemical composition. Learn More

A U.S. Patent Application for a "Virtual Transmitter for Bioreactor Automation System" was published by Finesse on August 22nd, 2019. The present invention includes a probe for measuring a condition in a bioreactor that can generate a signal. Measured conditions may include pH, DO, temperature, and pressure. The signal can travel to an electronic card capable of converting the signal from analog to digital and the card can then send the converted signal to a digital controller. The condition monitoring and control system eliminates hardware redundancies and increases versatility by then displaying the converted signal in a software-based virtual transmitter on a non-dedicated human machine interface. Learn More

A U.S. Patent on "Media for Culturing Stem Cells" was awarded to the Technion Research & Development Foundation on August 20th, 2019. The present invention is a well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. Learn More

A U.S. Patent for a "Process of Recycling Mixed PET Plastic Articles" was awarded to Carbios  on August 20th, 2019. The present invention relates to a method for recycling at least one mixed PET plastic article, the method comprising degrading at least PET of the mixed PET plastic article to monomers and/or oligomers using an enzyme and recovering the resulting monomers and/or oligomers. The method of the invention may be used for depolymerizing, simultaneously or sequentially at least two different polymers of the mixed PET plastic article, and/or for recycling at least two mixed PET plastic articles. Learn More

A U.S. Patent Application for an "Apparatus for Detecting pH and Dissolved Oxygen" was published by Scientific Industries  on August 15th, 2019 . The present invention is a cell culture monitoring system that may include a device having collimating and filtering components to elicit and detect fluorescence from a substrate located within a reaction vessel. The device may be used to detect changes in pH and dissolved oxygen levels in a liquid contained in the reaction vessel due to growth of living cells. Excitation light beams can be generated and collimated by a beam combiner and directed into the reaction vessel so as to be incident upon the substrate to cause the substrate to fluoresce. Learn More

A U.S. Patent for "Highly Concentrated Pharmaceutical Formulations" was awarded to Genentech  on August 13th, 2019 . The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-CD20 antibody, such as e.g. Rituximab, Ocrelizumab, or HuMab<CD20>, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-CD20 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. Learn More

A U.S. Patent for "Methods for Suspension Cultures of Human Pluripotent Stem Cells" was awarded to Janssen on August 13th, 2019. The present invention is an in vitro method of producing three-dimensional cell clusters of pluripotent stem cells comprising the steps of: a. treating pluripotent stem cells cultured in a planar adherent culture with a chelating agent or enzyme to release cell aggregates from the planar adherent culture; b. suspending the cell aggregates from the planar adherent culture in a culture medium in the presence of a Rho-kinase inhibitor without centrifuging the cell aggregates and without dissociating the cell aggregates to single cells. Learn More

on August 13th, 2019. The present invention  is a cell culture monitoring system are disclosed that may include a device having collimating and filtering components to elicit and detect fluorescence from a substrate located within a reaction vessel. The device may be used to detect changes in pH and dissolved oxygen levels in a liquid contained in the reaction vessel due to growth of living cells. Excitation light beams can be generated and collimated by a beam combiner and directed into the reaction vessel so as to be incident upon the substrate to cause the substrate to fluoresce.  Learn More

A U.S. Patent Application for a "Reversible Liquid Filtration System" was published by Sartorius Stedim on August 8th, 2019. The present invention is a reversible liquid filtration system for cell culture perfusion comprises: a bioreactor vessel (B4), for storing the cell culture (L4); a perfusion pump (P7), comprising a reciprocable element (P71) which is movable in opposing first and second pumping directions (dF, dR); a filter (F4); and first and second bi-directional valves (BV1, BV2), each selectively controllable between open and closed positions. The perfusion pump (P7), the filter (F4), and the first and second bi-directional valves...  Learn More

A U.S. Patent Application for a "Chromatography Method" was published by GE on August 8th, 2019. The present invention is a method of making affinity chromatography beads suitable for purification of immunoglobulin containing proteins or parts thereof, from contaminants that are larger than immunoglobulin containing proteins, the method comprising: a) providing allylated chromatography beads having an allylated shell layer and an allylated inner core wrapped around by the allylated shell layer; b) inactivating the allylated shell layer of the beads to form a lid of hydrolyzed allyl groups having a thickness of 1-8 .mu.m; c) activating the allylated inner core of the beads. Learn More

A U.S. Patent for "Reusable Aseptic Fluid Couplings" was awarded to Colder Products Company on August 6th, 2019. The present invention is an aseptic fluid coupling system comprising: a first coupling portion comprising: a first valve body defining a first longitudinal axis and a first end port; a first valve member disposed within the first valve body; and a cap that is releasably coupleable to the first valve body, the cap enclosing at least a portion of the first valve body while the cap is coupled to the first valve body; and a second coupling portion configured to releasably couple with the first coupling portion, comprising...  Learn More

A U.S. Patent for "Variable Diameter Bioreactors" was awarded to Lonza on August 6th, 2019. The present invention is a variable diameter bioreactor vessel that can include a first vessel section having a first diameter configured to hold a liquid media and biologic material and a second vessel section having a second diameter that is greater than the first diameter such that the liquid media can be increased from a first volume to a second volume within the vessel. In some aspects, the first vessel section can have an aspect ratio of greater than 0.3:1. In some aspects, the second vessel section can have an aspect ratio of greater than 0.3:1. Learn More

A U.S. Patent Application for "Binding Proteins, Including Antibodies, Antibody Derivatives and Antibody Fragments, that Specifically Bind CD154 and Uses Thereof" was published by UCB on August 1st, 2019. The present invention provides binding proteins, including antibodies, antibody derivatives and antibody fragments, that specifically bind a CD154 (CD40L) protein. This invention also provides a chimeric, humanized or fully human antibody, antibody derivative or antibody fragment that specifically binds to an epitope to which a humanized Fab fragment comprising a variable heavy chain sequence according to SEQ ID NO... Learn More

A U.S. Patent Application for a "Method of Culturing Eukaryotic Cells" was published by Genentech on August 1st, 2019. The present invention is an apparatus and method to maintain pH within a range conducive for cell growth in a bicarbonate-containing cell culture system without the addition of base. The method relies on the gas transfer characteristics of the bioreactor system to modulate the CO.sub.2 transfer to and from the cell culture such that the pH of the cell culture can be maintained within a desired range. Learn More

A U.S. Patent for "Large Scale Disposable Bioreactor" was awarded to Protalix on July 30th, 2019. The present invention is a reusable, disposable device for culturing plant tissues or cells including a non-rigid container having dimensions and gas exchange ports designed for maintaining oxygen saturation and shear forces suitable for culturing plant tissue or cells in 400 liters or more of culture medium is provided. Also provided are methods for producing a catalytically active human recombinant protein in a plant cell, using the disposable device of one of the embodiments of the instant specification. Learn More

A U.S. Patent for "Ion Exchange Membrane Chromatography" was awarded to Genentech on July 30th, 2019. The present invention are methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength... Learn More

A U.S. Patent Application for a "Method for Packing Chromatography Columns" was published by GE on July 25th, 2019. The present invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns; b) providing a plurality of chromatography resin aliquots; c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns; and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns. Learn More

A U.S. Patent Application for "Cell Culture Improvements" was published by AbbVie on July 25th, 2019. The present invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture. Learn More

A U.S. Patent Application for a "Computer Implemented Method for Creating a Fermentation Model" was published by Bayer on July 25th, 2019. The present invention is a computer-implemented method for model-based estimation of the state of a bioreaction with an organism in a bioreactor comprising: creating a model of a bioreaction with an organism, said model including a matrix L, kinetics of macroreactions and model parameters of calculated values, which comprises the following steps: a. defining selected metabolic pathways of the organism, their properties of stoichiometry and reversibility as background knowledge and calculating... Learn More

A U.S. Patent for a "Dual Wavelength Raman Probe with Spectral Concatenation of Data Filtration Systems" was awarded to Innovative Phototonic Solutions on July 23rd, 2019. The present invention is a compact dual-wavelength Raman probe using two laser sources each providing Raman excitation light at a different wavelength is disclosed causing Raman scattering in a fingerprint region associated with one excitation wavelength and causing Raman scattering in a stretch region, which are detected by the same detector array. Learn More

A U.S. Patent for "Perfusion Filtration Systems" was awarded to Artemis Biosystems on July 23rd, 2019. The present invention is a method of filtering a cell culture media comprising passing a cell culture media comprising mammalian cells and a virus through a plurality of tubular porous membranes of a filter elements enclosed within a casing, wherein each tubular porous membrane filter element comprises a plurality of sintered tubular ultra-high-molecular-weight polyethylene (UHMWPE) porous membranes, wherein at least 95% of the pores in each of the plurality of tubular porous membranes are between 5 .mu.m and -15 .mu.m in size... Learn More

A U.S. Patent Application for an "Aseptic Bioreactor Sampling System" was published by Trizell on July 18th, 2019. The present invention is an adherent cell culture bioreactor comprising: a container having an interior for containing liquid cell culture media and a solid support for adherent cell culture, the container interior in flowable communication with a first tube and a second tube, each of said tubes comprising an intake end in flowable communication with the container interior, and an output flush end and an output sample end, whereby liquid cell culture media can flow from the container interior into the intake end of the tube... Learn More