Patent Alert

A U.S. Patent for a "Horizontally Rocked Bioreactor System" was awarded to Bioreactor Sciences on July 9th, 2019. The present invention is a bioreactor system, comprising: a vessel assembly comprising: a tube positioned to lie along a longitudinal axis, the tube having ends; a baffle extending within the tube, the baffle extending along and contacting an inner wall of the tube along the longitudinal axis and extending to and contacting the ends of the tube, wherein a ratio of a major surface area of the baffle to a central cross-sectional area of the tube along the longitudinal axis is greater than about 0.45 and less than about 0.90...  Learn More

A U.S. Patent for a "Magnetophoresis System for Separation of Biological Particles" was awarded to Pablo Sanchez on July 9th, 2019.  The present invention is an apparatus for analyzing magnetically functionalized particles in a sample comprising: a chamber containing the magnetically functionalized particles in the sample; an analytic instrument communicating with the chamber to receive the magnetically functionalized particles in the sample for analysis; a magnetizable collection structure within the chamber for collecting magnetically functionalized particles from the sample.  Learn More

A U.S. Patent for "Systems and Methods for Adaptive Non-Linear Control of Process Systems" was awarded to Kyle Hunte on July 9th, 2019.  The present invention provides systems and methods for generating an adaptive nonlinear controller and utilizing the adaptive nonlinear controller to regulate the operation of nonlinear process systems. In particular, a method is provided for generating a control model by defining an objective function utilizing a target function that specifies the desired response of the system and a state-space model representing the dynamics of the non-linear system. Learn More

A U.S. Patent for a "Process for Reducing Subvisible Particles in a Pharmaceutical Formulation" was awarded to Regeneron on July 9th, 2019. The present invention provides a stable protein composition containing a surfactant and having less than 400 subvisible particles of 10 microns or greater diameter per container, or less than 10,000 subvisible particles of 2 microns or greater per container. A method of manufacturing such a stable protein composition is disclosed, which includes a unit of operation that removes or decreases an esterase activity that degrades the surfactant. The unit of operation may be hydrophobic interaction chromatography or filtration, mixed mode chromatography, or the like. Learn More

A U.S. Patent Application for an "Air Purification System" was published by AWE Solutions on July 4th, 2019.  The present invention is a system and process for purifying air. The system includes a housing and a plurality of inner and outer chambers. A solution of water and a biological reagent is configured to flow around the inner and outer walls of the inner and outer chambers while air is passed adjacent to this fluid flow. This causes an interaction between the air and the fluid solution to cleanse the air. The air is then discharged from the chamber. Learn More

A U.S. Patent Application for "Protein Purification"  was published by UCB on July 4th, 2019.  The present invention relates to a process for purification of a protein comprising a semi-continuous chromatography step whereby the flow-through is collected and re-loaded onto the chromatography matrix. Comprising the steps of a) in an operational chromatography cycle loading a first volume of a mixture containing the protein of interest from a first container to a chromatography matrix operated such that the protein binds to the chromatography matrix until 40% to 100% of the static binding capacity of the chromatography matrix is reached... Learn More

A U.S. Patent for "Processes for Starting Up and Operating Deep Tank Anaerobic Fermentation Reactors for Making Oxygenated Organic Compound from Carbon Monoxide and Hydrogen" was awarded to Synata on July 2nd, 2019. The present invention is a process for starting up and operating anaerobic, deep tank fermentation reactors including a process for anaerobic bioconversion of a gas substrate comprising carbon monoxide, hydrogen, and carbon dioxide in a reactor by contact of the gas substrate with an aqueous menstruum containing microorganisms suitable for converting the gas substrate to an oxygenated organic compound in the reactor. Learn More

A U.S. Patent Application for "Antibody Drug Conjugates" was published by Novartis on June 27th, 2019. The present invention discloses anti-P-cadherin antibodies, antigen binding fragments thereof, and antibody drug conjugates of said antibodies or antigen binding fragments, particularly antibody drug conjugates comprising anti-P-cadherin antibodies conjugated to auristatin analogs. The invention also relates to methods of treating cancer using the antibody drug conjugates. Also disclosed herein are methods of making the antibodies, antigen binding fragments, and antibody drug conjugates, and methods of using the antibodies and antigen binding fragments as diagnostic reagents. Learn More

A U.S. Patent Application for a "Method for Producing Recombinant Protein" was published by Spiber on June 27th, 2019. The present invention relates to a method for production of a recombinant protein using recombinant cells that express the recombinant protein under the control of an inducible promoter, the method including continuous culturing with addition of fresh culture medium to a portion of the culture solution in which the recombinant cells have been grown, wherein the recombinant cells whose expression of the recombinant protein has been induced are not reused. Learn More

A U.S. Patent Application for a "Method for Purification of Viruses or Virus Like Particles Using a Crosslinked Cellulose Hydrate Membrane" was published by Sartorius on June 27th, 2019. The present invention is a method for purifying viruses or virus-like particles from a solution, comprising the following steps: a) providing a mixture M composed of a solution A, which contains the viruses or the virus-like particles to be purified and impurities, and a solution B, which contains a polyalkylene glycol having two or three carbon atoms in the repeat unit, d) loading a crosslinked cellulose hydrate membrane with the mixture M from step... LEARN MORE

A U.S. Patent for a "Solventless Extraction Process" was awarded to DSM on June 25th, 2019. The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from micro-organisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained. Learn More

A U.S. Patent for "Conditionally Active Biological Proteins" was awarded to BioAtla on June 25th, 2019. The present invention is a method of preparing a conditionally active biological protein, the method comprising steps of: i) evolving the DNA which encodes a parent biological protein using one or more evolutionary techniques to create mutant DNAs; ii) expressing the mutant DNAs to obtain mutant biological proteins; iii) subjecting the mutant biological proteins to an assay under a first physiological condition selected from physiological conditions of a first location selected from the group. Learn More

A U.S. Patent Application for "Wireless Sensor Information Monitoring" was published by Lonza on June 20th, 2019. The present invention is a system, apparatus, and method for continuously monitoring data output from a wireless sensor on a mesh network at a frequency higher than mesh formation/propagation. For example, the system may include a wireless gateway, an amplifier coupled to the wireless gateway, a first antenna coupled to the amplifier, a second antenna coupled to the amplifier, and a wireless sensor. The wireless sensor may be a wireless temperature sensor configured to monitor the temperature of a cryopreserved material product. Learn More

A U.S. Patent Application for "Packed Bed Emulsification" was published by GE on June 20th, 2019. The present invention is a system for the production of polysaccharide droplets from a water-in-oil emulsion comprising, at least one chamber comprising an inlet side connectable to a source of water-in-oil emulsion containing droplets of an aqueous solution of a polysaccharide in an oil-based continuous phase, an outlet side, a packed bed of beads between the inlet side and the outlet side, and flow-producing means for providing a flow of water-in-oil emulsion through the packed bed to an outlet port, wherein the beads are hydrophilic beads. Learn More

A U.S. Patent for an "Apparatus, Systems and Methods for Growing Microorganisms" was awarded to Locus on June 18th, 2019. The present invention is a fermentation system that includes a fermentation tank, a plurality of sensors operable to measure a plurality of fermentation system parameters, a plurality of mechanisms operable to control the fermentation system parameters, and a controller in electronic communication with the sensors and with the mechanisms. The controller includes a memory, a receiver circuit, a transmitting circuit, and a control circuit. The memory stores a plurality of recipes each defining user operating instructions and parameter settings. Learn More

A U.S. Patent for "High Efficiency Continuous Countercurrent Tangential Chromatography" was awarded to Chromatan on June 18th, 2019. The present invention is a system, module and method for continuous or batch single-pass countercurrent tangential chromatography for bind/elute and negative chromatography applications. The system includes binding, washing, elution (for bind/elute), regeneration, and equilibration single-pass modules. The resin slurry flows in a continuous single pass at steady-state through each module, while corresponding buffers flow countercurrent to the slurry facilitating efficient product and impurity extraction... Learn More

A U.S. Patent Application for "Cell Culture Additives and Their Use for Increased Bioprotein Production from Cells" was published by Air Liquide on June 13th, 2019. The present invention relates to a method of increasing protein biomolecule production in which a) a cell that produces a heterologous protein biomolecule is cultured, and b) an Additive and/or a source of the Additive is added to the culture medium in an amount sufficient to (i) increase a total yield of the heterologous protein biomolecule secreted into the cell culture media and/or (ii) increase a specific cellular productivity of the heterologous protein biomolecule secreted into the cell culture media. Learn More

A U.S. Patent Application for a "Method of Producing a Recombinant Protein" was published by GSK on June 13th, 2019. The present invention relates to a recombinant signal sequence, and a fusion protein comprising the signal sequence and a recombinant protein. Methods of producing the recombinant protein using the recombinant signal sequence are described. In particular, the recombinant signal sequence provides a method for controlling the viscosity of the fermentation, and/or controlling basal (pre-induction) expression of the recombinant protein. Learn More

A U.S. Patent for a "Method for Producing a Product in a Continuous Cell Culture Fermentation Process"  was awarded to AGC Biologics on June 11th, 2019. The present invention is a method of producing a product selected from a biopolymer expressed by a cell, an intracellular or extracellular product produced by a cell or microorganism, a periplasmatic product produced by a cell or microorganism, a cell or a microorganism in a bioreactor in a chemostat fermentation process, wherein said bioreactor comprises: i) a first outlet having a separation device allowing impurities with a size below the size of the product, and medium to be removed... Learn More

A U.S. Patent Application for "End-to-End Cell Therapy Automation" was published by Lonza on June 6th, 2019. The present invention is a method for automated production of a genetically modified immune cell culture, the method comprising: (a) activating an immune cell culture with an activation reagent to produce an activated immune cell culture; (b) transducing the activated immune cell culture with a vector, to produce a transduced immune cell culture; (c) expanding the transduced immune cell culture; (d) concentrating the expanded immune cell culture of (c); and (e) harvesting the concentrated immune cell culture of (d)... Learn More

A U.S. Patent Application for a "Cell Retention Device and Method" was published by Boehringer Ingelheim on June 6th, 2019. The present invention relates to a cell retention device and method for use in a perfusion cell culture system where the cell retention device includes a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 .mu.m and having the ability to operate under perfusion cell culture conditions for up to 35 days without being clogged or losing its product sieving ability by more than 20%. Learn More

A U.S. Patent for "Aseptic Coupling Devices" was awarded to Colder Products Company on June 4th, 2019. The present invention is an aseptic coupling arrangement includes a first coupling device and a second aseptic coupling device. In one example, the first and second coupling devices are substantially similar, each having a main body having a front face and a fluid passageway therethrough. A first connecting feature on the main body of each coupling device may be provided for aligning and coupling the aseptic devices together. Each coupling device may also include a sealing member received in the main body and a membrane... Learn More

A U.S. Patent for "Fluid Manifold Systems" was awarded to Thermo Fisher on June 4th, 2019. The present invention is a fluid manifold system that includes a manifold having at least portions of opposing flexible sheets welded together to form a fluid flow path therebetween, a fluid inlet communicating with the fluid flow path. A plurality of receiving containers are in fluid communication with the fluid flow path of the manifold, each receiving container bounding a compartment. The receiving containers can be formed integral with the manifold by welding together a second portion of the opposing flexible sheets or can comprise separate containers that are coupled to the manifold. Learn More

A U.S. Patent for "Filter Systems for Separating Microcarriers from Cell Culture Solutions" was awarded to Thermo Fisher on May 28th , 2019. The present invention is a filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. An outlet port is attached to the container through which fluid flows out of the compartment. A filter is disposed within the compartment, the filter dividing the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber... Learn More

A U.S. Patent for a "Regulatable Promoter" was awarded to Lonza on May 28th, 2019. The present invention is a method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising a series of steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter... Learn More

A U.S. Patent for a "Method of Removing DNA from Biotechnological Products" was awarded to BASF on May 28th, 2019. The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or biotransformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45.degree. C. to 55.degree. C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA. Learn More

A U.S. Patent for a "High-Cell Density Fed-Batch Fermentation Process for Producing Recombinant Protein" was awarded to Pfizer on May 28th, 2019. The present invention includes methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions for use in the methods of the present invention, are provided. Learn More

A U.S. Patent Application for an "Automated Bioprocess Development" was awarded to TAP on May 23rd, 2019. The present invention is a method for automating process development in a bioprocessing environment. The method comprising: executing a first experiment run according to a set of parameters; retrieving a first real-time set of data of the experiment nm while the experiment run is being executed; retrieving a second real-time set of data of an experiment run being executed in parallel, analysing the retrieved first real-time set of data and the second real-time set of data to determine an adjusted set of parameters; and, modifying, based on the analysis... Learn More

A U.S. Patent for a "Bioreactor with Condenser" was awarded to Sartorius Stedim on May 21st, 2019. The present invention is a bioreactor with a vessel having at least one gas dissipation duct for gas discharge, the orifice of the gas dissipation duct being connected to a hydrophobic sterile filter and to a condenser arranged between them and having condensation surfaces, a turbulence generator for generating a turbulent flow is arranged in the gas dissipation duct in the region of the condenser. Learn More

A U.S. Patent for a "Computer-Implemented Method for Creating a Fermentation Model" was awarded to Bayer on May 21st, 2019. The present invention is a computer-implemented method for process control of a bioreaction with an organism comprising: creating a model of a bioreaction with an organism, said model including a matrix L, kinetics of macroreactions and model parameters of calculated values, which comprises the following steps: a. defining selected metabolic pathways of the organism, their properties of stoichiometry and reversibility as background knowledge and calculating elementary modes from this input; b. combining the elementary modes. Learn More

A U.S. Patent for a "Flexible Manufacturing System" was awarded to Xoma on May 21st, 2019. The present invention is a unitary structure adapted to be disposed within a housing, comprising: at least one controlled air, sealable, sterilizable cleanroom having an entry point and an exit point; a cleanroom entry portion coupled to and selectively isolated from the cleanroom, the cleanroom entry portion for controlling personnel flow to the cleanroom via the entry point; a cleanroom exit portion coupled to and selectively isolated from the cleanroom and separated from the cleanroom entry portion, the cleanroom exit portion for controlling personnel flow... Learn More

A U.S. Patent Application for "Particles for Use in Acoustic Standing Wave Processes" was published by FloDesign Sonics on May 16th, 2019. The present invention is a method for concentrating particles in a primary fluid at a first location, comprising: flowing a fluid mixture comprising the particles and the primary fluid through an acoustophoretic device that comprises: an acoustic chamber through which the fluid mixture flows; and an ultrasonic transducer including a piezoelectric material that can be driven to create an acoustic wave in the acoustic chamber; and driving the ultrasonic transducer to create the acoustic wave; wherein the acoustic wave concentrates the particles at the first location. Learn More

A U.S. Patent Application for " Automated Control of Cell Culture Using Raman Spectroscopy"  was published by Lonza on May 16th, 2019. The present invention provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different cell lines producing different mAbs. Developed predictive models, qualified using an independent cell line not used in calibration... Learn More

A U.S. Patent for "Methods of Reducing Level of One or More Impurities in a Sample During Protein Purification" was awarded to MilliporeSigma on May 14th, 2019. The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product. Learn More

A U.S. Patent for a "Syringe Containing Stable Aqueous Formulations of Adalimumab" was awarded to Coherus Biosciences on May 14th, 2019. The present invention relates to aqueous pharmaceutical compositions suitable for long-term storage of adalimumab (including antibody proteins considered or intended as "biosimilar" or "bio-better" variants of commercially available adalimumab), methods of manufacture of the compositions, methods of their administration, and kits containing the same. Learn More

A U.S. Patent for a "High Pressure Bioreactor" was awarded to Carnegie Institution on May 7th, 2019. The present invention relates generally to an integrated system, apparatus and method that allows for the continuous culturing of microorganisms under high pressure conditions and at a wide range of temperatures. More specifically, the system is configured to be gas tight and operate under aerobic or anaerobic conditions. The system is also configured to permit periodic sampling of the incubated organisms under such conditions with minimal physical and chemical disturbance inside the reactor and minimal impacts of shear forces on the collected biomass. Learn More

A U.S. Patent for "Recipient for Cell Cultivation" was awarded to Pall on May 7th, 2019. The present invention is a system for cell cultivation, comprising a recipient having an inner compartment for cell growth, the recipient comprising: an outer tubular wall extending in a longitudinal direction, the outer tubular wall delimiting an outer boundary of the inner compartment in a radial direction; first and second ends delimiting the inner compartment at the first outer end and the second outer end of the outer tubular wall; a packing in the inner compartment comprising a fiber matrix... Learn More

A U.S. Patent for " Highly Concentrated Pharmaceutical Formulations"  was awarded to Genentech  on May 7th, 2019. The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-CD20 antibody, such as e.g. Rituximab, Ocrelizumab, or HuMab<CD20>, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-CD20 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. Learn More

A U.S. Patent for " Cell Culture Additives and Their Use for Increased Bioprotein Production from Cells"  was awarded to Air Liquide  on May 7th, 2019. The present invention relates to a method of increasing protein biomolecule production in which a) a cell that produces a heterologous protein biomolecule is cultured, and b) an Additive and/or a source of the Additive is added to the culture medium in an amount sufficient to (i) increase a total yield of the heterologous protein biomolecule secreted into the cell culture media and/or (ii) increase a specific cellular productivity of the heterologous protein biomolecule secreted into the cell culture media. Learn More

A U.S. Patent for an " Apparatus, Method and System for Recording at Least One Variable During a Biological and Chemical Process"  was awarded to PreSens  on May 7th, 2019. The present invention is an apparatus, a method and a system for the parallelized recording of at least one variable during a biological/chemical process are disclosed. A matrix, which has at least one container and which can be positioned on a measurement carrier, is provided to accommodate the liquid samples. A measuring unit, which comprises a controllable radiation source for electromagnetic radiation and at least one sensor for detecting electromagnetic radiation, is fixedly disposed in or on the measurement carrier. Learn More

A U.S. Patent Application for "Direct Selection of Cells Expressing High Levels of Heteromeric Proteins Using Glutamine Synthetase Intragenic Complementation Vectors" was published by Amgen on May 2nd, 2019. The present invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express polypeptides, in particular heteromultimeric polypeptides. Learn More

A U.S. Patent Application for "Compositions and Methods for Regulating Cell Osmolarity" was published by Genentech on May 2nd, 2019. The present invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE). Learn More

A U.S. Patent for " Abrasion Resistant Film for Biocontainers"  was published by MilliporeSigma on April 30th, 2019. The present invention is a biocontainer film enhanced with an abrasion resistant or "cut-proof" substrate. Such substrates can be combined with current biocontainer materials, via various techniques of embedding, coextrusion or laminating, to maintain the cleanliness and low extractables already validated for biotech manufacturing. The substrate of choice may be constructed from materials known to be more resistant to abrasion and sharp razor type cuts or from materials oriented in such a way to prevent puncture to occur. Learn More

A U.S. Patent Application for " Cation Exchange Chromatography Methods"  was published by Bristol- Myers Squibb  on April 25th, 2019. The present invention provides improved methods of protein purification using CEX chromatography. Such methods generally comprise the steps of: contacting a protein of interest (e.g., an antibody) with a cation exchange resin at a first pH, that is less than the pI of the most acidic isoform of the protein of interest, such that the protein of interest binds to the resin; washing the cation exchange resin at a second pH that is greater than the first pH, but less than the p1 of the most acidic isoform of the protein of interest... Learn More

A U.S. Patent Application for a " Method and Apparatus for Quantifying Solutions Comprised of Multiple Analytes"  was published by Finesse on April 25th, 2019. The present invention is a multi-analyte sensor system based on hollow core photonic bandgap fiber and Raman anti-Stokes spectroscopy. The system includes: i) an inlet to introduce an analyte sample into an analyzer chamber which analyzer includes; ii) a measurement system to derive the anti-Stokes spectral peaks and/or spectra of the sample; iii) a set of reference calibrants corresponding to the analytes of which the sample is primarily comprised; iv) a second inlet to introduce said calibrants into the analyzer. Learn More

A U.S. Patent Application for " Inhibition of Protein Degradation for Improved Production" was published by Lonza  on April 25th , 2019.  The present invention discloses methods and compositions useful for evaluating, selecting, identifying, or making a cell or cell line that has improved production capacity for generating higher yields of products and/or improved capacity to produce higher quality products. Products, as described herein, can include a polypeptide that is endogenously expressed by the cell, a recombinant polypeptide that is not endogenously expressed, or a non-naturally occurring recombinant polypeptide. Learn More

A U.S. Patent for a " Magnetic Mixing System and Method" was awarded to Sani-Tech West on April 23rd , 2019. The present invention is a mixing system typically for use in a container for mixing its contents, the mixing system including a cap unit, an extension unit and a magnetic mixing unit that is attached to the cap unit by the extension unit. The magnetic mixing unit can be folded to permit insertion and/or removal of the system via a mouth of the container. The extension unit and magnetic mixing unit connect via a hinge formed by upper and lower hinge portions that meet at a pivot point.  Learn More

A U.S. Patent for a " Chromatography Method"  w as awarded to GE on April 23rd , 2019. The present invention is a method for purification of immunoglobulin containing proteins or parts thereof, from contaminants that are larger than immunoglobulin containing proteins, the method comprising: a) contacting an immunoglobulin containing sample with agarose chromatography beads comprising a lid consisting of agarose modified with hydrolyzed allyl groups and having a thickness of 1-8 .mu.m with a defined pore size and an inner core of defined pore size consisting of agarose modified with an affinity ligand. Learn More

A U.S. Patent for a " Micro Titre Plate"  w as awarded to GE on April 23rd , 2019. The present invention is a micro titre plate comprising a number of wells disposed in a plurality of columns and rows, the wells being filled with the same type of separation matrix, wherein the separation matrix in the wells of the plate has a series of volumes V.sub.1, V.sub.2, . . . V.sub.n distributed according to a specific pattern that does not obey logarithmic or a single degree freedom linear relationship and same separation matrix volume is provided in all the wells of either the same column or the same row in the plate...  Learn More

A U.S. Patent Application for " In Situ Raman Spectroscopy Systems and Methods for Controlling Process Variables in Cell Cultures" w as published by Regeneron on April 18th, 2019. The present invention provides in situ Raman spectroscopy methods and systems for monitoring and controlling one or more process variables in a bioreactor cell culture in order to improve product quality and consistency. The methods and systems utilize in situ Raman spectroscopy and chemometric modeling techniques for real-time assessments of cell cultures, combined with signal processing techniques, for precise continuous feedback and model predictive control... Learn More

A U.S. Patent for a " Cost-Effective Raman Probe Assembly for Single-Use Bioreactor Vessels"  was awarded to Kaiser Optical  on April 16th, 2019. The present invention consists of systems and methods used to couple an optical sampling probe to a port in a single-use bioreactor bag for in-process monitoring. A combination of re-useable and disposable components maintain precision while reducing costs. A disposable barb with an integral window, received by the port of the reaction vessel, is coupled to a re-useable optic component with a focusing lens. A separate focus alignment tool is used to set the lens position to a precise focal point before placement of the optic component into the barb. Learn More

A U.S. Patent for " Stackable Chromatography Column Modules and Flow Control Blocks" was awarded to  GE  on April 16th, 2019. The present invention discloses a flow control block for a stack of chromatography column modules, as well as a stack of chromatography modules comprising at least one flow control block. The flow control block is in a first position or configuration capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in parallel and in a second position or configuration it is capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in series. Learn More

A U.S. Patent Application for "Monoclonal Anti-IL-1RACP Antibodies" was published by MAB Discovery on April 11th, 2019. The present invention is a monoclonal antibody that specifically binds the interleukin 1 receptor type 1, or an antigen binding fragment thereof, comprising: a) a heavy chain variable region (VH) comprising CDR1H, CDR2H and/or CDR3H, wherein the CDR1H region comprises an amino acid sequence selected from the group of SEQ ID NO: 155-231, wherein the CDR2H region comprises an amino acid sequence selected from the group of SEQ ID NO: 232-308, and wherein the CDR3H region comprises an amino acid sequence selected... Learn More

A U.S. Patent for " Particle Analyzing Systems and Methods Using Acoustic Radiation Pressure" was awared to Thermo Fisher  on April 9th, 2019.  The present invention is a particle analyzing system, comprising: a flow cell configured to receive a sample stream that comprises a plurality of particles; an acoustic device configured to acoustically focus at least some of the particles within the flow cell; a fluidics system configured to hydrodynamically focus at least some of the plurality of particles within the flow cell; a controller configured to automate an aspect of the particle analyzing system operation; and, a monitor configured to monitor at least some of the plurality of particles... Learn More

A U.S. Patent for a " Method for Retrovirus Removal"  was published by MilliporeSigma on April 9th, 2019. The present invention is a method for removing retroviruses from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention is disclosed. Retroviruses are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a retrovirus LRV greater than about 6, and the nanofiber(s) has a diameter from about 10 nm to about 100 nm. The filtration medium can be in the form of a fibrous electrospun polymeric nanofiber liquid filtration medium mat. Learn More

A U.S. Patent for a " Bioreactor Arrangement, Shaking Device and Method for Irradiating a Medium in a Bioreactor"  was published by Sartorius Stedim on April 9th, 2019. The present invention is a bioreactor arrangement that has a bioreactor and an illuminating body for illuminating a medium in a reactor interior. The illuminating body has at least one emission surface via which light, reflected by at least one end surface into the reactor interior, is emitted. The bioreactor is formed as a container having a transparent wall. A shaking device has a reactor holder that can be in oscillated, for a bioreactor with a transparent wall. Learn More

A U.S. Patent Application for " Single-Pass Filtration Systems and Processes"  was published by MilliporeSigma on April 4th, 2019. The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass TFF assemblies, scalable single-pass TFF assemblies and adaptable modular single-pass TFF assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass TFF assembly and for cleaning a TFF assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass TFF assembly. Learn More

A U.S. Patent for a " Flow Regulator as well as Method for Setting a Predefinable Volume Flow"  was awarded to Levitronix on April 2nd, 2019. The present invention is a flow regulator that includes a first sensor determining a first actual value of a first flow parameter of the fluid, a pre-pressure regulator setting a predefinable pre-pressure at a pre-pressure outlet of the pre-pressure regulator, and a regulating pump. The fluid is capable of being supplied to the pre-pressure regulator at a plant pressure in an operating state via an inflow line and a pre-pressure inlet and capable of being conducted further to the regulating pump at the pre-pressure. Learn More

A U.S. Patent for a " Fluid Transfer Device"  was awarded to MilliporeSigma on April 2nd, 2019. The present invention is a disposable device for the sterile transfer of fluids, such as liquids or gases, includes a body, a bore formed through at least a portion of the interior of the body, and a linearly moveable plunger contained within the bore. The body is formed from a rotating section and a stationary section. Partial rotation of the rotating section around a portion of the stationary section and the plunger drives the plunger linearly within the bore, opening and closing the device. The plunger includes at least a first and a second opening, and a fluid channel in the interior of the plunger. Learn More

A U.S. Patent Application for an " Optically Clear Sealable Petri Dish Bioreactor"  was published by Autobiologic on March 28th, 2019.  The present invention is a petri dish bioreactor that can allow a standard Petri dish to be converted to a microscopically observable bioreactor. One advantage of this conversion is to provide an inexpensive device that can be used for cell culture, observation, transportation, and storage in the laboratory and clinical setting. Further, by creating a sealed bioreactor chamber accessible by ports, the device can reduce cross contamination and allow for the maintenance of a controlled microenvironment within the bioreactor. Learn More

A U.S. Patent Application for a " Scalable Primate Pluripotent Stem Cell Aggregate Suspension Culture and Differentiation Thereof"  was published by Viacyte on March 28th, 2019. The present invention relates to suspension cell aggregate compositions that are essentially serum and feeder-free and methods for differentiating the cell aggregate suspensions. It further relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof. Learn More

A U.S. Patent Application for an "Improved Fermentation Process" was publsihed by Lonza on March 28th, 2019. The present invention is a method for producing a recombinant protein of interest (POI) comprising: (a) culturing cells in a cell culture medium to express said POI by adding a feed comprising at least one substrate to said cell culture, (b) applying a feeding strategy based on calculating, setting and optionally controlling the specific substrate uptake rate q.sub.S of the cells during the induction phase and/or production phase of the POI, wherein q.sub.S is set to be close to the maintenance rate of the cell culture; and (c) isolating said POI from the cell culture. Learn More

A U.S. Patent for a "Method for the Production of Protein Complexes and Vaccine Compositions Comprising the Same" was awarded to Immunobiology on March 26th, 2019. The present invention relates to methods for the production of heat shock protein complexes for use in vaccine compositions. In particular, there is provided a method for increasing the level and immunogenicity of heat shock protein complexes produced in cells by subjecting the cells to specific stress-inducing stimuli. The invention further extends to the use of heat shock protein complexes produced. Learn More

A U.S. Patent for a "Bioreactor Container and Integrity Check Method for Bioreactor Containers" was awarded to Sartorius Stedim on March 26th, 2019. The present invention is a bioreactor container comprising: an at least locally flexible wall that has a fluid-impermeable inner sheet and an at least locally structured outer sheet, the structured outer sheet being configured to separate the fluid-impermeable sheet from an object outside the bioreactor container so that the object from outside the bioreactor container does not come in direct mechanical contact with the fluid-impermeable inner sheet and so that a leak in the fluid-impermeable inner sheet cannot be sealed by the object. Learn More

A U.S. Patent Application for "Production of Extracellular Vesicles in Single-Cell Suspension Using Chemically-Defined Cell Culture Media" was published by Codiak BioSciences on March 21st, 2019. The present invention are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal-derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. Learn More

A U.S. Patent Application for a "Process for Manipulating the Level of Glycan Content of a Glycoprotein" was published by Amgen on March 21st, 2019. The present invention is a recombinant protein produced by a method for manipulating the fucosylated glycan content on a recombinant protein comprising inoculating a bioreactor with mammalian host cells expressing the recombinant protein, culturing the mammalian host cells in a serum-free, chemically defined cell culture medium; wherein the cell culture medium includes from 10 to 100 ppb copper and from 50 to 1000 nM manganese. Learn More

A U.S. Patent Application for the "Control of Protein Glycosylation by Culture Medium Supplementation and Cell Culture Process Parameters was published by Biogen on March 21st, 2019. The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. Learn More

A U.S. Patent for a " Batch Authoring Tool and Bioreactor Control System"  was awarded to GE on March 19 th , 2019. The present invention is a bioreactor control system comprising a batch server for controlling a bioreactor system in response to a recipe, a batch software system for generating a control sequence for the bioreactor system via the batch server for implementing the recipe, and a recipe conversion system that inputs the recipe into the batch software system. Preferably, the recipe conversion system reads a word processor file containing the recipe and interprets styles applied to text in the word processor file as indicating equipment and phases of the recipe to the recipe conversion system. Learn More

A U.S. Patent for "Purification of Proteins" was awarded to MilliporeSigma on March 19th, 2019. The present invention relates to a selectively soluble polymer capable of binding to a desired molecule in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc). Learn More

A U.S. Patent Application for "Animal Protein-Free Media for Cultivation of Cells" was published by Baxalta on March 14th, 2019. The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes. Learn More

A U.S. Patent for "Overexpression of N-Glycosylation Pathway Regulators to Modulate Glycosylation of Recombinant Proteins" was awarded to Amgen on March 12th, 2019. The present invention is a method of regulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process, comprising: transfecting a mammalian host cell to overexpress a protein that is involved in an N-glycosylation pathway, wherein the protein involved in the N-glycosylation pathway is selected from the group...wherein the mammalian cell culture is perfused using alternating tangential flow (ATF), and wherein the recombinant protein is harvested and purified. Learn More

A U.S. Patent for "Composite Sensor Assemblies for Single Use Bioreactors" was awarded to Finesse on March 12th, 2019. The present invention is a composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises: i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate being fusibly sealable to the bioreactor vessel at a hole in the wall thereof; ii) a generally opaque polymeric monitoring sensor assembly including electrical, and/or optical measurement components. Learn More

A U.S. Patent for a "Compounded Media Powder Formulation and Method of Preparation of Liquid Medium for Cell Culture" was awarded to Bayer on March 12th, 2019. The present invention provides a compounded cell culture medium powder formulation comprising: a basal medium powder and a cell culture media supplement, wherein the cell culture media supplement comprises and one or more salts; one or more growth factors; one or more inorganic ions; an amino acid supplement comprising one or more of asparagine, glutamine, histidine, and serine; one or more buffers; and one or more anti-foaming agents. Learn More

A U.S. Patent Application for "Methods for Continuously Inactivating a Virus During Manufacture of a Protein" was published by Boehringer Ingelheim on March 7th, 2019. The present invention refers to methods for continuously inactivating a virus during manufacture of a protein are provided. Steps include (1) combining, at a single predetermined volumetric ratio, a composition including the protein, and a composition including a virus-inactivation reagent, to obtain a treatment composition having a predetermined property for inactivation of a virus; (2) transferring the treatment composition to a treatment vessel; (3) incubating the treatment composition in the treatment vessel at predetermined conditions... Learn More

A U.S. Patent Application for a "Method for Determining the InVivo Comparability of a Biologic Drug and a Reference Drug" was published by Bioanalytix on March 7th, 2019. The present invention provides a mass-spectroscopic approach for assessing and determining the in vivo comparability of a candidate biologic molecule to a reference biologic molecule. The results can be presented in the form of an in vivo comparability profile, which can serve as a development tool, e.g., as a guide or target for the development of biologies, biosimilars, or gene therapy-based drugs. Learn More

A U.S. Patent for a "Disposable Bioreactor Condenser Bag and Filter Heater" was awarded to GE on March 5th, 2019. The present invention is a system for condensing moisture in a moist gas stream entering or leaving a bioreactor includes a condenser container, the condenser container comprising: an outer wall surface and an inner wall surface, the inner wall surface defining an interior chamber for holding a fluid; and a first fitment attached to the outer wall surface and forming a first port configured to allow the moist gas stream to flow into the interior chamber; a second fitment attached to the outer wall surface and forming a second port configured to allow a dried gas to flow from the interior chamber and out of the second port... Learn More

A U.S. Patent for a "Method of Producing a Polypeptide or Virus of Interest in a Continuous Cell Culture" was awarded to Baxalta on March 5th, 2019. The present invention is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device. Learn More

A U.S. Patent Application for a "Chromatography Ligand Comprising Domain C from Staphylococcus Aureus Protein A for Antibody Isolation" was published by GE on February 28th, 2019. The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. Learn More

A U.S. Patent Application for an "Electronically Controlled Diaphragm Pump" was published by Stobbe Pharma on February 28th, 2019. The present invention is an electronically controlled diaphragm pump system that includes a pump housing with a drive gas chamber, a fluid chamber separated by a diaphragm, gas pressure means for providing a drive gas pressure in response to a control signal, and gas under-pressure means connected to a gas outlet port for providing a gas outlet under-pressure or sucking of gas in response to a control signal. Learn More

A U.S. Patent for a "Process for the Purification of a Growth Factor Protein" was awarded to Octapharma on February 26th, 2019. The present invention is a method of purifying a Growth Factor Protein in a purification sequence employing chromatography is provided. The method comprises performing at least one chromatography step using a multimodal resin, binding the Growth Factor Protein to said resin at a pH from 4 to 6.2, and eluting the Growth Factor Protein at a pH in the range of from 5.5 to 6.5. The elution of Growth Factor Protein is improved by addition of arginine and/or NaCl in the eluting buffer. Optionally the multimodal resin step is followed which results in a purity of the product greater than 90%. Learn More

A U.S. Patent for a "Distributed Perfusion Bioreactor System for Continuous Culture of Biological Cells" was awarded to the University of Massachusetts on February 26th, 2019. The present invention is a distributed bioreactor system in which a plurality of modular bioreactors are operated in parallel to produce and maintain a biological cell culture. A central or nurse perfusion bioreactor produces and maintains a cell culture and transfers portions of the cell culture to a plurality of modular peripheral perfusion bioreactors, each of which produces and maintains the cell culture in turn. In order to prevent contamination and facilitate segregation of particular peripheral bioreactors... Learn More

A U.S. Patent Application for a "Method for Chromatography Reuse" was published by Genentech on February 21st, 2019. The present invention provides methods for cleaning or regenerating a chromatography material for reuse. The methods of the invention can be used for cleaning or regenerating chromatography columns for reuse in the large-scale manufacture of multiple polypeptide products. Learn More

A U.S. Patent for "Pre-Sterilized Syringe System and Method of Use" was awarded to Paw Bioscience on February 19th, 2019. The present invention is a pre-sterilized fluid sampling syringe is provided which includes a locking vacuum syringe, 3-way valve, check valve and thermoplastic inlet tubing capable of both aseptic welding and direct connections. The syringe is assembled with components and rendered sterile in its packaging. The packaging permits manipulation of the syringe by the user such that at time of use a vacuum can be generated in the syringe barrel by pulling and locking the plunger into a fully withdrawn position. Learn More

A U.S. Patent for "Single-Pass Filtration Systems and Processes" was awarded to MilliporeSigma on February 19th, 2019. The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration solutions, scalable single-pass tangential flow filtration assemblies, and adaptable modular single-pass tangential flow filtration solutions. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. Learn More

A U.S. Patent for a "Fluid Flow Cell Including a Spherical Lens" was awarded to MarqMetrix on February 19th, 2019. The present invention is a flow cell device including a spherical optical element is disclosed. The spherical lens can be sealed to the body of the flow cell device in a manner that provides external optical access to a fluid in an analysis region of a flow path through the flow cell device. The seal can be provided by an elastomer, a polymer, or a deformable metal. The disposition of the spherical lens to the flow path enables in situ optical analysis of the fluid. An optical analysis device can be removably connected to the flow cell device to provide the optical analysis. Learn More

A U.S. Patent Application for "Dry Powder Cell Culture Products and Methods of Production Thereof" was published by Thermo Fisher on February 14th, 2019. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. Learn More

A U.S. Patent Application for "Perfusion Bioreactor and Method for Using Same to Perform a Continuous Cell Culture" was published by Corning on February 14th, 2019. The present invention is a perfusion bioreactor comprising: a vessel having a housing comprised at least in part of gas permeable membrane, at least one opening and a cavity; at least one lid attachable to the vessel to cover the at least one opening; a porous membrane disposed within the cavity to divide the cavity into an inner compartment and an outer compartment; a fresh media port extending through the vessel or the at least one lid; a spent media port extending through the vessel or the at least one lid; and, a mixer. Learn More

A U.S. Patent for "Temperature Shift for High Yield Expression of Polypeptides in Yeast and Other Transformed Cells" was awarded to AlderBio Holdings on February 12th, 2019. The present invention is a method of producing a desired antibody in yeast cells, comprising: (a) culturing at a first temperature yeast cells comprising one or more genes that encode said desired antibody; and (b) culturing said yeast cells at a second temperature and allowing said yeast cells to produce said desired antibody; wherein said first temperature is between 27.5 degrees C. and 28.5 degrees C. and said second temperature is between 30 degrees C. and 31 degrees C. Learn More

A U.S. Patent Application for "Decreasing Lactate Level and Increasing Polypeptide Production by Downregulating the Expression of Lactate Dehydrogenase and Pyruvate Dehydrogenase Kinase" was published by Genentech on February 7th, 2019. The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). Learn More

A U.S. Patent Application for "Method of Actuation of an Alternating Tangential Flow Diaphragm Pump" was published by Repligen on February 7th, 2019. The present invention is a control unit that includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Learn More

A U.S. Patent for "Methods and Devices Used for Redundant Sterile Filtration" was awarded to EMD Millipore on February 5th, 2019. The present invention is a redundant filtration system that includes at least one barrier filter as a process filter, the barrier filter having both hydrophilic and hydrophobic paths, allowing both fluid and gas permeability. The need for vents is eliminated, as is an intermediate drain filter. The device also can be dried in series. The filtration system includes a network of conduits and receptacles, the network receiving liquid raw and/or starting material at one end, conducting it through the process stream defined thereby, and producing the desired liquid product at another end. Learn More

A U.S. Patent for an "Apparatus and Method for Making a Sterile Connection of Flexible Tubing" was awarded to GE on February 5th, 2019. The present invention is an apparatus for substantially sterile connection of flexible tubing, including at least one tubing holder, which is adapted to receive one or more lengths of flexible tubing and which is also equipped with one or more sterilant applicators. The sterilant applicator is adapted to apply a sterilant to the length of tubing when the tubing length is received in the tubing holder. At least one cutter, which is movable in relation to the tubing holder and adapted to cut the length of tubing. Learn More

A U.S. Patent Application for a "Device and Method for Drawing a Liquid from a Process Tank" was published by Endress Hauser on January 31st, 2019. The present invention discloses a device for drawing a liquid from a process tank to be protected from contamination, including: a first receptacle for receiving the liquid withdrawn from the process tank; a first liquid line connecting the process tank with the first receptacle; and at least one first valve assembly disposed in the first liquid line and designed to block or release liquid transport through the first liquid line. The device includes at least one pressure sensor for detecting a pressure within the first receptacle. Learn More

A U.S. Patent for a "Semi-Automated Sampling System for Aseptic Sampling was awarded to GE on January 29th, 2019. The present invention is a semi-automated sampling assembly configured for aseptic sampling at one or more instances from a sample source having a biological inoculum is provided. The semi-automated sampling assembly includes a sampling conduit, a recovery conduit, one or more sampling kits, and a pumping device. The sampling conduit includes a first port and a second port, where the first port of the sampling conduit is configured to be operatively coupled to the sample source. Learn More

A U.S. Patent for a "Method of Culturing Eukaryotic Cells" was awarded to Genentech on January 29th, 2019. The present invention is a method for culturing Chinese hamster ovary (CHO) cells comprising: (i) providing cell culture inoculant comprising CHO cells in about 6 L of a bicarbonate-containing culture liquid to a vessel to achieve a target cell density of about 7.5.times.10.sup.5 CHO cells/mL, wherein said vessel has a volume of about 50 L and has walls that encapsulate said cell culture and a gas phase head space above said cell culture, and wherein said vessel comprises at least one port that provides an entrance and an egress of gas to and from said head space. Learn More

A U.S. Patent for an "Affinity Chromatography Matrix" was awarded to GE Healthcare on January 29th, 2019. The present invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, where in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine. Learn More

A U.S. Patent for "High Density Cell Banking Methods" was awarded to Genzyme on January 29th, 2019. The present invention is a non-centrifugal method for producing a high-density frozen mammalian cell bank, the method comprising: a) culturing mammalian cells in a perfusion bioreactor to a first cell density by continuously removing growth medium from a culture and replacing with fresh growth medium, wherein the bioreactor is coupled to a cell retention system comprising an alternating tangential flow filtration system
including a filter. Learn More

A U.S. Patent Application for "Production of Proteins in Glutamine-Free Cell Culture Media" was published by
Genentech on January 24th, 2019. The present invention is based, at least in part, on the unexpected finding that not only can recombinant proteins be produced in a mammalian host cell using a glutamine-free production medium without any significant adverse effect, in fact the use of a glutamine-free medium in the production phase significantly increases cell viability, culture longevity, specific productivity and/or the final recombinant protein titer. Learn More

A U.S. Patent for "Apparatus for Connection of Thermoplastic Tubing" was awarded to GE on January 22nd, 2019. The present invention discloses an apparatus for aligning and supporting a first and a second length of thermoplastic tubing, preparatory to and during cutting and welding of said lengths of tubing, that includes a first holder arranged to accommodate portions of the first and second lengths of tubing in a first and a second elongated holding space respectively, a second holder arranged to accommodate portions of the first and second lengths of tubing in a third and a fourth elongated holding space respectively, and movable... Learn More