Patent Alert

A U.S. Patent Application for "Production of Extracellular Vesicles in Single-Cell Suspension Using Chemically-Defined Cell Culture Media" was published by Codiak BioSciences on March 21st, 2019. The present invention are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal-derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. Learn More

A U.S. Patent Application for a "Process for Manipulating the Level of Glycan Content of a Glycoprotein" was published by Amgen on March 21st, 2019. The present invention is a recombinant protein produced by a method for manipulating the fucosylated glycan content on a recombinant protein comprising inoculating a bioreactor with mammalian host cells expressing the recombinant protein, culturing the mammalian host cells in a serum-free, chemically defined cell culture medium; wherein the cell culture medium includes from 10 to 100 ppb copper and from 50 to 1000 nM manganese. Learn More

A U.S. Patent Application for the "Control of Protein Glycosylation by Culture Medium Supplementation and Cell Culture Process Parameters was published by Biogen on March 21st, 2019. The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. Learn More

A U.S. Patent for a " Batch Authoring Tool and Bioreactor Control System"  was awarded to GE on March 19 th , 2019. The present invention is a bioreactor control system comprising a batch server for controlling a bioreactor system in response to a recipe, a batch software system for generating a control sequence for the bioreactor system via the batch server for implementing the recipe, and a recipe conversion system that inputs the recipe into the batch software system. Preferably, the recipe conversion system reads a word processor file containing the recipe and interprets styles applied to text in the word processor file as indicating equipment and phases of the recipe to the recipe conversion system. Learn More

A U.S. Patent for "Purification of Proteins" was awarded to MilliporeSigma on March 19th, 2019. The present invention relates to a selectively soluble polymer capable of binding to a desired molecule in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc). Learn More

A U.S. Patent Application for "Animal Protein-Free Media for Cultivation of Cells" was published by Baxalta on March 14th, 2019. The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes. Learn More

A U.S. Patent for "Overexpression of N-Glycosylation Pathway Regulators to Modulate Glycosylation of Recombinant Proteins" was awarded to Amgen on March 12th, 2019. The present invention is a method of regulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process, comprising: transfecting a mammalian host cell to overexpress a protein that is involved in an N-glycosylation pathway, wherein the protein involved in the N-glycosylation pathway is selected from the group...wherein the mammalian cell culture is perfused using alternating tangential flow (ATF), and wherein the recombinant protein is harvested and purified. Learn More

A U.S. Patent for "Composite Sensor Assemblies for Single Use Bioreactors" was awarded to Finesse on March 12th, 2019. The present invention is a composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises: i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate being fusibly sealable to the bioreactor vessel at a hole in the wall thereof; ii) a generally opaque polymeric monitoring sensor assembly including electrical, and/or optical measurement components. Learn More

A U.S. Patent for a "Compounded Media Powder Formulation and Method of Preparation of Liquid Medium for Cell Culture" was awarded to Bayer on March 12th, 2019. The present invention provides a compounded cell culture medium powder formulation comprising: a basal medium powder and a cell culture media supplement, wherein the cell culture media supplement comprises and one or more salts; one or more growth factors; one or more inorganic ions; an amino acid supplement comprising one or more of asparagine, glutamine, histidine, and serine; one or more buffers; and one or more anti-foaming agents. Learn More

A U.S. Patent Application for "Methods for Continuously Inactivating a Virus During Manufacture of a Protein" was published by Boehringer Ingelheim on March 7th, 2019. The present invention refers to methods for continuously inactivating a virus during manufacture of a protein are provided. Steps include (1) combining, at a single predetermined volumetric ratio, a composition including the protein, and a composition including a virus-inactivation reagent, to obtain a treatment composition having a predetermined property for inactivation of a virus; (2) transferring the treatment composition to a treatment vessel; (3) incubating the treatment composition in the treatment vessel at predetermined conditions... Learn More

A U.S. Patent Application for a "Method for Determining the InVivo Comparability of a Biologic Drug and a Reference Drug" was published by Bioanalytix on March 7th, 2019. The present invention provides a mass-spectroscopic approach for assessing and determining the in vivo comparability of a candidate biologic molecule to a reference biologic molecule. The results can be presented in the form of an in vivo comparability profile, which can serve as a development tool, e.g., as a guide or target for the development of biologies, biosimilars, or gene therapy-based drugs. Learn More

A U.S. Patent for a "Disposable Bioreactor Condenser Bag and Filter Heater" was awarded to GE on March 5th, 2019. The present invention is a system for condensing moisture in a moist gas stream entering or leaving a bioreactor includes a condenser container, the condenser container comprising: an outer wall surface and an inner wall surface, the inner wall surface defining an interior chamber for holding a fluid; and a first fitment attached to the outer wall surface and forming a first port configured to allow the moist gas stream to flow into the interior chamber; a second fitment attached to the outer wall surface and forming a second port configured to allow a dried gas to flow from the interior chamber and out of the second port... Learn More

A U.S. Patent for a "Method of Producing a Polypeptide or Virus of Interest in a Continuous Cell Culture" was awarded to Baxalta on March 5th, 2019. The present invention is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device. Learn More

A U.S. Patent Application for a "Chromatography Ligand Comprising Domain C from Staphylococcus Aureus Protein A for Antibody Isolation" was published by GE on February 28th, 2019. The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. Learn More

A U.S. Patent Application for an "Electronically Controlled Diaphragm Pump" was published by Stobbe Pharma on February 28th, 2019. The present invention is an electronically controlled diaphragm pump system that includes a pump housing with a drive gas chamber, a fluid chamber separated by a diaphragm, gas pressure means for providing a drive gas pressure in response to a control signal, and gas under-pressure means connected to a gas outlet port for providing a gas outlet under-pressure or sucking of gas in response to a control signal. Learn More

A U.S. Patent for a "Process for the Purification of a Growth Factor Protein" was awarded to Octapharma on February 26th, 2019. The present invention is a method of purifying a Growth Factor Protein in a purification sequence employing chromatography is provided. The method comprises performing at least one chromatography step using a multimodal resin, binding the Growth Factor Protein to said resin at a pH from 4 to 6.2, and eluting the Growth Factor Protein at a pH in the range of from 5.5 to 6.5. The elution of Growth Factor Protein is improved by addition of arginine and/or NaCl in the eluting buffer. Optionally the multimodal resin step is followed which results in a purity of the product greater than 90%. Learn More

A U.S. Patent for a "Distributed Perfusion Bioreactor System for Continuous Culture of Biological Cells" was awarded to the University of Massachusetts on February 26th, 2019. The present invention is a distributed bioreactor system in which a plurality of modular bioreactors are operated in parallel to produce and maintain a biological cell culture. A central or nurse perfusion bioreactor produces and maintains a cell culture and transfers portions of the cell culture to a plurality of modular peripheral perfusion bioreactors, each of which produces and maintains the cell culture in turn. In order to prevent contamination and facilitate segregation of particular peripheral bioreactors... Learn More

A U.S. Patent Application for a "Method for Chromatography Reuse" was published by Genentech on February 21st, 2019. The present invention provides methods for cleaning or regenerating a chromatography material for reuse. The methods of the invention can be used for cleaning or regenerating chromatography columns for reuse in the large-scale manufacture of multiple polypeptide products. Learn More

A U.S. Patent for "Pre-Sterilized Syringe System and Method of Use" was awarded to Paw Bioscience on February 19th, 2019. The present invention is a pre-sterilized fluid sampling syringe is provided which includes a locking vacuum syringe, 3-way valve, check valve and thermoplastic inlet tubing capable of both aseptic welding and direct connections. The syringe is assembled with components and rendered sterile in its packaging. The packaging permits manipulation of the syringe by the user such that at time of use a vacuum can be generated in the syringe barrel by pulling and locking the plunger into a fully withdrawn position. Learn More

A U.S. Patent for "Single-Pass Filtration Systems and Processes" was awarded to MilliporeSigma on February 19th, 2019. The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration solutions, scalable single-pass tangential flow filtration assemblies, and adaptable modular single-pass tangential flow filtration solutions. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. Learn More

A U.S. Patent for a "Fluid Flow Cell Including a Spherical Lens" was awarded to MarqMetrix on February 19th, 2019. The present invention is a flow cell device including a spherical optical element is disclosed. The spherical lens can be sealed to the body of the flow cell device in a manner that provides external optical access to a fluid in an analysis region of a flow path through the flow cell device. The seal can be provided by an elastomer, a polymer, or a deformable metal. The disposition of the spherical lens to the flow path enables in situ optical analysis of the fluid. An optical analysis device can be removably connected to the flow cell device to provide the optical analysis. Learn More

A U.S. Patent Application for "Dry Powder Cell Culture Products and Methods of Production Thereof" was published by Thermo Fisher on February 14th, 2019. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. Learn More

A U.S. Patent Application for "Perfusion Bioreactor and Method for Using Same to Perform a Continuous Cell Culture" was published by Corning on February 14th, 2019. The present invention is a perfusion bioreactor comprising: a vessel having a housing comprised at least in part of gas permeable membrane, at least one opening and a cavity; at least one lid attachable to the vessel to cover the at least one opening; a porous membrane disposed within the cavity to divide the cavity into an inner compartment and an outer compartment; a fresh media port extending through the vessel or the at least one lid; a spent media port extending through the vessel or the at least one lid; and, a mixer. Learn More

A U.S. Patent for "Temperature Shift for High Yield Expression of Polypeptides in Yeast and Other Transformed Cells" was awarded to AlderBio Holdings on February 12th, 2019. The present invention is a method of producing a desired antibody in yeast cells, comprising: (a) culturing at a first temperature yeast cells comprising one or more genes that encode said desired antibody; and (b) culturing said yeast cells at a second temperature and allowing said yeast cells to produce said desired antibody; wherein said first temperature is between 27.5 degrees C. and 28.5 degrees C. and said second temperature is between 30 degrees C. and 31 degrees C. Learn More

A U.S. Patent Application for "Decreasing Lactate Level and Increasing Polypeptide Production by Downregulating the Expression of Lactate Dehydrogenase and Pyruvate Dehydrogenase Kinase" was published by Genentech on February 7th, 2019. The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). Learn More

A U.S. Patent Application for "Method of Actuation of an Alternating Tangential Flow Diaphragm Pump" was published by Repligen on February 7th, 2019. The present invention is a control unit that includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Learn More

A U.S. Patent for "Methods and Devices Used for Redundant Sterile Filtration" was awarded to EMD Millipore on February 5th, 2019. The present invention is a redundant filtration system that includes at least one barrier filter as a process filter, the barrier filter having both hydrophilic and hydrophobic paths, allowing both fluid and gas permeability. The need for vents is eliminated, as is an intermediate drain filter. The device also can be dried in series. The filtration system includes a network of conduits and receptacles, the network receiving liquid raw and/or starting material at one end, conducting it through the process stream defined thereby, and producing the desired liquid product at another end. Learn More

A U.S. Patent for an "Apparatus and Method for Making a Sterile Connection of Flexible Tubing" was awarded to GE on February 5th, 2019. The present invention is an apparatus for substantially sterile connection of flexible tubing, including at least one tubing holder, which is adapted to receive one or more lengths of flexible tubing and which is also equipped with one or more sterilant applicators. The sterilant applicator is adapted to apply a sterilant to the length of tubing when the tubing length is received in the tubing holder. At least one cutter, which is movable in relation to the tubing holder and adapted to cut the length of tubing. Learn More

A U.S. Patent Application for a "Device and Method for Drawing a Liquid from a Process Tank" was published by Endress Hauser on January 31st, 2019. The present invention discloses a device for drawing a liquid from a process tank to be protected from contamination, including: a first receptacle for receiving the liquid withdrawn from the process tank; a first liquid line connecting the process tank with the first receptacle; and at least one first valve assembly disposed in the first liquid line and designed to block or release liquid transport through the first liquid line. The device includes at least one pressure sensor for detecting a pressure within the first receptacle. Learn More

A U.S. Patent for a "Semi-Automated Sampling System for Aseptic Sampling was awarded to GE on January 29th, 2019. The present invention is a semi-automated sampling assembly configured for aseptic sampling at one or more instances from a sample source having a biological inoculum is provided. The semi-automated sampling assembly includes a sampling conduit, a recovery conduit, one or more sampling kits, and a pumping device. The sampling conduit includes a first port and a second port, where the first port of the sampling conduit is configured to be operatively coupled to the sample source. Learn More

A U.S. Patent for a "Method of Culturing Eukaryotic Cells" was awarded to Genentech on January 29th, 2019. The present invention is a method for culturing Chinese hamster ovary (CHO) cells comprising: (i) providing cell culture inoculant comprising CHO cells in about 6 L of a bicarbonate-containing culture liquid to a vessel to achieve a target cell density of about 7.5.times.10.sup.5 CHO cells/mL, wherein said vessel has a volume of about 50 L and has walls that encapsulate said cell culture and a gas phase head space above said cell culture, and wherein said vessel comprises at least one port that provides an entrance and an egress of gas to and from said head space. Learn More

A U.S. Patent for an "Affinity Chromatography Matrix" was awarded to GE Healthcare on January 29th, 2019. The present invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, where in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine. Learn More

A U.S. Patent for "High Density Cell Banking Methods" was awarded to Genzyme on January 29th, 2019. The present invention is a non-centrifugal method for producing a high-density frozen mammalian cell bank, the method comprising: a) culturing mammalian cells in a perfusion bioreactor to a first cell density by continuously removing growth medium from a culture and replacing with fresh growth medium, wherein the bioreactor is coupled to a cell retention system comprising an alternating tangential flow filtration system
including a filter. Learn More

A U.S. Patent Application for "Production of Proteins in Glutamine-Free Cell Culture Media" was published by
Genentech on January 24th, 2019. The present invention is based, at least in part, on the unexpected finding that not only can recombinant proteins be produced in a mammalian host cell using a glutamine-free production medium without any significant adverse effect, in fact the use of a glutamine-free medium in the production phase significantly increases cell viability, culture longevity, specific productivity and/or the final recombinant protein titer. Learn More

A U.S. Patent for "Apparatus for Connection of Thermoplastic Tubing" was awarded to GE on January 22nd, 2019. The present invention discloses an apparatus for aligning and supporting a first and a second length of thermoplastic tubing, preparatory to and during cutting and welding of said lengths of tubing, that includes a first holder arranged to accommodate portions of the first and second lengths of tubing in a first and a second elongated holding space respectively, a second holder arranged to accommodate portions of the first and second lengths of tubing in a third and a fourth elongated holding space respectively, and movable... Learn More

A U.S. Patent for "Methods for Viral Inactivation and Other Adventitious Agents" was awarded to Genentech on January 22nd, 2019. The present invention is a method for inactivating virus or adventitious agents in a mammalian cell culture media while the media maintains suitability for mammalian cell culture, said method comprising a) adjusting the pH of the cell culture media, wherein the cell culture media has a pH 5.0 to pH 6.9 prior to HTST treatment, b) subjecting the cell culture media to HTST treatment, and c) raising the pH of the cell culture media to between 6.9 and 7.2 after HTST treatment. Learn More

A U.S. Patent for a "Virtual Transmitter for Bioreactor Automation System" was awarded to Finesse on January 22nd, 2019. The present invention relates to an environmental condition monitoring and control system employing a software-based virtual transmitter for a bioreactor or mixer. The system includes a probe for measuring a condition in a bioreactor that can generate a signal. Measured conditions may include pH, DO, temperature, and pressure. The signal can travel to an electronic card capable of converting the signal from analog to digital and the card can then send the converted signal to a digital controller. Learn More

A U.S. Patent Application for "Particle Sensor with Interferent Discrimination" was published by BugLab on January 17th, 2019. The present invention provides methods and devices to measure particle suspension concentrations in the presence of potential interferents. Particle back-scatter readings are taken at light wavelengths that are absorbed by the medium before interacting with surrounding objects. Source-detector spacings are minimized compared to the mean absorbance path length of light, thereby maximizing the range of sensitivity to particle concentration and enabling discrimination against potentially interfering particles, such as bubbles, is provided. Learn More

A U.S. Patent Application for "Suspension Culture of Human Embryonic Stem Cells" was published by Asterias Biotherapeutics on January 17th, 2019. The present invention is a method for culturing undifferentiated human pluripotent stem (hPS) cells in suspension, the method comprising: a) suspending undifferentiated hPS cells in a cell culture medium comprising basic fibroblast growth factor (bFGF); b) maintaining the hPS cells substantially undifferentiated while culturing in suspension; c) changing the cell culture medium periodically; and d) optionally splitting the culture from time to time, so as to reduce the density of the hPS cells in culture; wherein the hPS cells are not grown attached to a solid substrate. Learn More

A U.S. Patent for "Methods and Compositions for Increased Safety of Stem Cell-Derived Populations" was awarded to MIT on January 15th, 2019. The present invention is a method for reducing residual pluripotent stem cells in an in vitro differentiated cell population, comprising culturing an in vitro differentiated cell population at an oxygen partial pressure that is less than 142 mmHg for at least 1 day and less than 10 days, and harvesting the differentiated cell population after the culturing step; wherein the harvested cell population comprises less than 1 pluripotent stem cell per 10.sup.3 cells, wherein 50% or more of the cells in the in vitro differentiated cell population are positive for at least one differentiation marker before the culturing step. Learn More

A U.S. Patent for a "Bioreactor for the Cultivation of Mammalian Cells" was awarded to Lonza on January 15th, 2019. The present invention is a scalable bioreactor for the cultivation of mammalian cells, the bioreactor comprising: a biocompatible tank or vessel having a volume of between 500 liters and 20,000 liters and at least one top impeller and at least one bottom impeller, wherein the at least one top impeller has a power number between 0.1 and 0.9, wherein the at least one top impeller has a flow number between 0.4 and 0.9, wherein the at least one bottom impeller has a power number between 0.5 and 0.9, wherein an impeller spacing between the at least one top impeller. Learn More

A U.S. Patent Application for "Methods for Purification of Recombinant AAV Vectors" was published by Genzyme on January 10th, 2019. The present invention is a method for isolating a population of recombinant adeno-associated virus (rAAV) particles from in-process impurities in a feedstream, comprising the steps of: (a) (i) contacting a feedstream containing the rAAV particles with an apatite chromatography medium in the presence of polyethylene glycol (PEG), wherein the rAAV particles bind to the apatite chromatography medium; and (ii) eluting the rAAV particles bound to the apatite chromatography medium with an elution buffer... Learn More

A U.S. Patent Application for "Overexpression of N-Glycosylation Pathway Regulators to Modulate Glycosylation of Recombinant Proteins" was published by Amgen on January 10th, 2019. The present invention is a method of regulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process, comprising: transfecting a mammalian host cell to overexpress a protein that is involved in an N-glycosylation pathway, wherein the protein involved in the N-glycosylation pathway is selected from the group consisting of N-acetyl-glucosaminyltransferase-1 (encoded by Mgat1); N-acetyl-glucosaminyltransferase-2 (encoded by Mgat2); a UDP-Galactose transporter encoded by Slc35a2... Learn More

A U.S. Patent for "Use of Vibrational Spectroscopy for DNA Content Inspection" was awarded to 1087 Systems on January 8th, 2019. The present invention is a cytometry system, comprising: a microfluidic channel with a measurement volume in which a cell in a sample fluid is introduced; at least one laser source configured to emit at least one light at one or more wavelengths towards the cell resulting in a detectable change in the light; wherein the at least one laser source is further configured to emit at a wavelength corresponding to the resonant absorption for the cell DNA... Learn More

A U.S. Patent for "Performance Enhancing Genetic Variants of E. coli" was awarded to the Regents of the University of California on January 8th, 2019. The present invention provides mutant Escherichia coli cells that contain one or more mutations in one or more of the rpoB, hns/tdk, corA, ygaZ, iap, metL, ygeW, and pyrE/rph genes (exemplified in Table 2A and 2B), which confer on the mutant in minimal media the phenotype of increased level of growth and/or increased glucose uptake rate and/or increased acetate production rate and/or increased biomass yield, compared to a control E. coli (such as wild type E. coli) that lacks the one or more mutations in the one or more genes. Learn More

A U.S. Patent for "Filter Element and Filtration Assembly for Biopharmaceutical Applications" was awarded to 3M on January 8th, 2019. The present invention are filter elements or cells and filtration assemblies or capsules for purifying and/or clarifying fluids used during various bioprocessing applications, for example, chromatography or depth filtration, and processes for making and using the same. A filter element comprises a media pack comprising two sides and a separator element located between the two sides, at least one side comprising a filtration media, wherein an inner edge periphery and/or an outer edge periphery of the filtration media comprise a bond directly to a portion of the separator element. Learn More

A U.S. Patent for "Reduced-Viscosity Concentrated Protein Formulations" was awarded to Genentech and Novartis on January 1st, 2019. The present invention concerns a method of lowering the viscosity of concentrated protein composition by: (1) increasing the total ionic strength of the formulation through the addition of salts or buffer components; or (2) altering the pH of the formulation to be lower (.apprxeq.4.0 to .apprxeq.5.3) or elevated (.apprxeq.6.5 to .apprxeq.12.0), without significantly compromising stability or biological activity. Accordingly, the invention concerns methods and means for reducing the viscosity of concentrated protein formulations, primarily to ensure easy manipulation before and during administration to a patient. Learn More

A U.S. Patent for a "Method for Connecting Modular Mobile Rooms" was awarded to G-CON Manufacturing on December 25th, 2018. The present invention is a method and a connector unit for connecting two or more structures wherein at least one of the two or more structures being connected is a mobile structure validatable for pharmaceutical manufacturing or patient care. The connector unit comprises of an alignment system, a sealing, and a fixation system. The method for using the connector unit comprises of aligning the two or more structures to dock the two or more structures together, sealing the two or more structures, or one or more structures and an environment air-tight with a seal or a sealing system... Learn More

A U.S. Patent for "Use of an Amino Acid Auxotrophy Cured Prokaryotic Strain for the Recombinant Production of a Polypeptide" was awarded to Roche on December 18th, 2018. The present invention is a method for producing a polypeptide in a prokaryotic cell, comprising the step of cultivating a prokaryotic cell comprising one or more nucleic acids encoding the polypeptide in a chemically defined minimal growth medium and recovering the polypeptide from the prokaryotic cell or the periplasm of the prokaryotic cell or from the medium, wherein the prokaryotic cell is an amino acid auxotrophy cured prokaryotic cell... Learn More

A U.S. Patent for a "Tri-Color Dual Glucose and Oxygen Sensors and Methods of Preparing and Using Them" was awarded to the Arizona Board of Regents on December 18th, 2018. The present invention relates to an optical fluorescence sensor comprising a probe for sensing glucose, an intra-reference probe, and a matrix. The present disclosure also relates to an optical fluorescence dual sensor comprising a probe for sensing glucose having two boronic acid moieties, a probe for sensing oxygen comprising modified porphyrin, an intra-reference probe that is rhodamine-based, and a matrix. The present disclosure additionally relates to methods of preparing these sensors and methods of using them. Learn More

A U.S. Patent Application for "Deep Eutectic Solvents and/or Ionic Liquids as Feed Media" was published by Merck KGaA on December 13th, 2018. The present invention is a liquid feed medium comprising a deep eutectic solvent and/or an ionic liquid. It has been found that deep eutectic solvents and/or ionic liquids can be used as highly concentrated liquid feeds for cell culture in bioreactors. Deep eutectic solvents and/or ionic liquids are per se liquid and do not need to be dissolved by the addition of a solvent which has no other effect on the cell culture than diluting it. At least one component of the deep eutectic solvent and/or ionic liquid is, favorably, a nutrient component... Learn More

A U.S. Patent Application for "Chromatography Columns, Systems and Methods" was published by GE on December 13th, 2018. The present invention is an axial flow chromatography column comprising: a housing comprising a side wall; axially spaced first and second end units positioned opposed to each other are separated by said side wall; a first filter which is adjacent to said first end unit and a second filter which is adjacent to said second end unit wherein said first and second filters together with the side wall define an enclosed bed space for containing a bed of particulate medium. Learn More

A U.S. Patent for "Bioreactor Systems with Tube Supports" was awarded to Thermo Fisher on December 11th, 2018. The present invention is a bioreactor system that includes a support housing having an interior surface bounding a compartment, the support housing having a sidewall with an opening extending therethrough and communicating with the compartment. A rack is secured to the support housing in alignment with the opening. An elongated first shaft has a first end and an opposing second end, the first end of the first shaft being secured to the rack so that the second end of the first shaft projects outwardly away from the sidewall of the support housing. Learn More

A U.S. Patent for "Control of pH and Dissolved Gas in Medium" was awarded to Kuros Biosciences on December 11th, 2018. The present invention is a method and device to adjust the concentration of at least two gasses in a fluid, wherein the method involves adjusting the concentration of a first gas in the fluid by adjusting the concentration of a second gas in the fluid wherein the first and second gases are kept separate until dissolved in the fluid. Learn More

A U.S. Patent Appliction for a "Cell Culture Medium" was published by Boehringer Ingelheim on December 6th, 2018. The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. Learn More

A U.S. Patent for "Methods of Cell Culture" was awarded to Momenta on December 4th, 2018. The present invention is a method of producing an adalimumab preparation having a target value of one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans, the method comprising: (a) providing a cell genetically engineered to express adalimumab; (b) culturing the cell in a culture medium under conditions in which the cell expresses the adalimumab; and (c) harvesting a preparation of the adalimumab produced by the cell that meets the target value of the one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans... Learn More

A U.S. Patent Application for a "Method for Controlling Continuous Chromatography and Multi-Column Chromatography Arrangement" was published by the Karlsruher Institute on November 29th, 2018. The present invention is a method of controlling at least one multi-column chromatography arrangement for the continuous process of purifying biopharmaceuticals are provided. The methods include introducing a first multi-component mixture into a column of the multi-column chromatography arrangement; detecting at least one multivariate signal by at least one detector; calculating at least one process parameter on the basis of the multivariate signal... Learn More

A U.S. Patent Application for "Production of Viruses in Cell Culture" was published by the University of Georgia on November 29th, 2018. The present invention relates to methods of replicating viruses in vitro. In particular, the invention relates to a genetically modified population of cells, and/or a population of cells treated with an exogenous compound, wherein the cells are capable of producing more virus than cells lacking the genetic modification and/or lacking treatment with the exogenous compound. The invention also relates to methods of producing populations of such cells, as well as the use of the viruses obtained to prepare vaccine compositions. Learn More

A U.S. Patent for "Temperature Shift for High Yield Expression of Polypeptides in Yeast and Other Transformed Cells" was awarded to Alder Bio on November 27th, 2018. The present invention is a method of producing a recombinant multi-subunit complex in yeast cells, comprising: (a) culturing at a first temperature yeast cells comprising one or more genes that encode said multi-subunit complex; and (b) culturing said yeast cells at a second temperature and allowing said yeast cells to produce said multi-subunit complex; wherein said first temperature is between 27.5.degrees C and 28.5.degrees C and said second temperature is between 30 degrees C and 31 degrees C. Learn More

A U.S. Patent for a "Process for Protein Production" was awarded to Novo Nordisk on November 27th, 2018. The present invention relates to a process for the production of a haemostasis protein by continuous perfusion culturing of a cell culture in suspension, said cell culture expressing said haemostasis protein into said culture suspension, wherein the cell culture flows across a filter module, which filter module leads to a harvest port, the filter module having a mesh size of from 0.1 to 2.9 .mu.m allowing passage across of the haemostasis protein and wherein the flow across the filter module is an alternating tangential flow. The invention also relates to a protein produced by the process of the invention. Learn More

A U.S. Patent for "Devices, Systems and Methods for Automated Cell Culturing" was awarded to GE on November 20th, 2018. The present invention is a multi-scalable bioreactor, which comprises a culture vessel for seeding and culturing cells by adding a cell-culture media, wherein the culture vessel comprises at least a side wall and a bottom surface, a specific heat transfer area and a specific gas transfer area; wherein the culture vessel is configured to accommodate the cell-culture media volume up to 10 liters, and wherein the specific heat transfer area and the specific gas transfer area are independent of cell-culture media volume... Learn More

A U.S. Patent for "Protein Purification Using Bis-Tris Buffer" was awarded to Sanofi on November 20th, 2018. The present invention is a method for purifying a monoclonal antibody or antigen-binding fragment thereof from solution comprising: (a) a first chromatography step comprising: passing said solution over a first Protein A affinity chromatography column; and eluting a crude protein eluent from the first chromatography column using a first elution buffer; and (b) a second chromatography step comprising: passing the crude protein eluent obtained at the end of step (a) over a second chromatography column; and recovering purified monoclonal antibody... Learn More

A U.S. Patent Application for a "Mixing Device for Mixing the Content of a Bioreactor, Comprising a Synchronization Mechanism" was published by Sartorius Stedim on November 15th, 2018. The present invention relates to a system comprising a mixing device for mixing the content of a bioreactor. The system comprises at least one movement device for initiating a mixing movement in the bioreactor or in a holder for receiving a bioreactor and at least one sensor that can be arranged in or on the bioreactor for receiving at least one physiological or physical measurement variable. The mixing device or the bioreactor further comprises a sensor or transmitter for generating a synchronization measurement variable. Learn More

A U.S. Patent Application for "Methods for the Harvesting of Cell Cultures" was published by Millenium Pharma, Now Takeda, on November 15th, 2018. The present invention provides methods for optimization of the harvest process by clarification of cell samples using centrifugation and depth filtration. The present invention provides methods for the determination of the optimal ratio of Q/.SIGMA. for the centrifugation step of a harvest process of a cell culture. The present invention provides methods for the determination of the number of particles and the size of the particles in the centrate of a centrifugation step of a harvest process of a cell culture by the use of imaging technology. Learn More

A U.S. Patent for a "Method for Recycling Plastic Products" was awarded to Carbios on November 13th, 2018. The present invention is a method for recycling at least one plastic product comprising at least partially semi-crystalline polyethylene terephthalate (PET) and at least one other additive, the method comprising: providing, without sorting, a mixture of plastic products coming from plastic waste collection and comprising said at least one plastic product; selecting a degrading enzyme suitable for degrading the at least partially semi-crystalline PET of the at least one plastic product; depolymerizing the at least partially semi-crystalline PET of the plastic product to monomers... Learn More

A U.S. Patent Application for a "Probe Port and Related Container System" was published by Life Technologies on November 8th, 2018. The present invention is a container system that includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. The probe port also includes a flange coupled to the tubular member and radially outwardly projecting therefrom, the flange being secured to the flexible bag... Learn More

A U.S. Patent for a "Fluid Mixing System with Steady Support" was awarded to Life Technologies on November 6th, 2018. The present invention is a fluid mixing system that includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment. A first rotational assembly includes a first casing mounted to the first end of the flexible bag and a first hub rotatably mounted to the first casing. A second rotational assembly includes a second casing mounted to the second end of the flexible bag and a second hub rotatably mounted to the second casing. A connector has a first end coupled with the first hub and an opposing second end. Learn More

A U.S. Patent for a "Modified Serum-Free Cell Culture Medium" was awarded to AbbVie on November 6th, 2018. The present invention provides improved salt-free basal growth media for use in mammalian cell culture. The invention includes a serum-free cell culture medium comprising Part A, Part B, and Part C, wherein Part A consists essentially of a modified basal medium which excludes the following components: sodium bicarbonate, a buffer, mono-basic sodium phosphate, di-basic sodium phosphate, an osmolarity regulator, a surfactant, and monosaccharide glucose; Part B consists essentially of an inorganic iron source. Learn More

A U.S. Patent for a "Coriolis Flow Meter for Measuring Properties of a Fluid and Method Therefor" was awarded to GE on November 6th, 2018. The present invention is a coriolis flow meter for measuring one or more properties of a fluid is described herein which involves a modular configuration, and includes a fluid flow sub-system and a mechanical oscillator sub-system, both functionally separate, and are coupled in a closed loop arrangement, such that the flow conduit is not directly vibrated, and instead receives induced oscillations from the mechanical oscillator sub-system. The Coriolis flow meter is useful for high purity applications, as well as for the bioprocessing applications. Learn More

A U.S. Patent Application for a "Novel Cell Line Screening Method" was published by Larix Biosciences on November 1st, 2018. The present invention invention provides a novel cell line development method useful to screen for recombinant protein production. The method utilizes a membrane-anchored reporter or an intracellular reporter residing in the expression vector for a gene of interest to facilitate initial cell selection by FACS or MACS. A switching mechanism can be used to delete the reporter from the chromosome by providing an appropriate DNA recombinase, which turns the selected cells into production cells that secrete the protein of interest without co-expression of the reporter. Learn More

A U.S. Patent Application for "Recombinant Virus Production Using Mammalian Cells in Suspension" was published by AGTC on November 1st, 2018. The present invention is a method for producing recombinant AAV viral particles in a mammalian cell comprising: co-infecting a mammalian cell capable of growing in suspension with a first recombinant herpesvirus comprising a nucleic acid encoding an AAV rep and an AAV cap gene each operably linked to a promoter; and (ii) a second recombinant herpesvirus comprising a gene of interest, and a promoter operably linked to said gene of interest; and allowing the virus to infect the mammalian cell; thereby producing recombinant AAV viral particles in a mammalian cell. Learn More

A U.S. Patent Application for a "Method and Chromatography Medium" was published by GE on October 25th, 2018. The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media. Learn More

A U.S. Patent for a "Method and Apparatus for Antibody Production and Purification" was published by Biovest on October 9th, 2018. The present invention pertains to methods and apparatus for the production and purification of cell products, such as immunoglobulins. One aspect of the invention is an integrated cell culture and purification apparatus for the growth and maintenance of cells and the harvest and purification of cell products, such as immunoglobulins. Thus, the apparatus integrates a cell culture function with a purification function. Other aspects of the invention pertain to an automated method for producing immunogenic compositions such as vaccines. Learn More

A U.S. Patent for a "Cell Culturing System for Cultivating Adherent Cells and Liquid Supply Interface Comprising a Cell Culture Container" was published by Hamilton on October 9th, 2018. The present invention relates to improvements in cell culturing systems comprising at least one cell culture container for collection and supply, i.e. particularly for cultivating with the goal of propagation, of adherent cells therein, comprising a nutrient medium reservoir for supplying the adherent cells in the cell culture container with nutrients, having a cleaning fluid reservoir for cleaning fluid flow paths or/and flow areas with the cleaning fluid, and comprising a liquid supply interface for coupling to the cell culture container. Learn More

A U.S. Patent for a "Device for Delivery of Sample Fluid" was published by GE on October 9th, 2018. The present invention relates to a device for delivery of fluid, said device comprising at least two fluid ducts of flexible material, each of which in one end can be connected to a fluid source and in the opposite end is connected to a manifold having an inlet end and an outlet end, each of said fluid ducts comprising a pinch valve for closing and opening the duct. According to the invention a check valve is disposed in at least one of said fluid ducts in the end thereof connected to the manifold. Learn More

A U.S. Patent for a "Contaminant Removal Method" was awarded to CSL on October 2nd, 2018. The present invention is a method for purifying Apo A-I is provided including the steps of providing a solution comprising Apo A-I and guanidine hydrochloride and filtering the solution through a filter having a pore size in a range from 15 nrn to 35 nm to thereby reduce viral contamination of the Apo A-I. An Apo A-f preparation is provided having at least a 12 log LRV (log reduction value) for a parvovirus; and/or at least 9 log LRV for a non-enveloped virus; and/or at least 8.5 log LRV for a lipid enveloped virus. Learn More

A U.S. Patent for a "Device, System and Process for Modification or Concentration of Cell-Depleted Fluid"
was awarded to Repligen on September 25th, 2018. The present invention is a product concentration device that utilizes a reservoir connected to a hollow-fiber filter element where the reservoir can serve as a container for filtrate emanating from another filtering device, such that product in the reservoir can be stored, concentrated and/or further processed as desired. Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump. Learn More

A U.S. Patent for "Vertical Wheel Bioreactors" was awarded to PBS on September 25th, 2018. The present invention is a pneumatic bioreactor includes a vessel containing a fluid to be mixed and at least one mixing device driven by gas pressure. A first embodiment includes a floating impeller that rises and falls in the fluid as gas bubbles carry it upward to the surface where the gas is then vented, permitting the impeller to sink in the fluid. The floating impeller may be tethered to a second impeller with a flexible member and pulley. The mixing speed is controlled with electromagnets in the vessel acting upon magnetic material in the impeller or its guides... Learn More

A U.S. Patent for a "Fuser and Sealer Integrated System" was awarded to GE on September 25th, 2018. The present invention is an integrated system for fusing and sealing a plurality of tubes, the integrated system comprises: at least one fusing unit, each fusing unit comprising at least two holders, wherein each holder is capable of receiving and holding a portion of a tube of the plurality of tubes, a cutting blade for cutting a portion of the tube received in each holder of the at least two holders simultaneously, and a driving assembly comprises a first cam member operatively connected to the cutting blade and a second cam member operatively connected... Learn More

A U.S. Patent Application for "Automated Batch Data Analysis" was published by Lonza on September 20th, 2018. The present invention is a system for automated batch data analysis. The system for automated batch data analysis includes, for example, at least one system component and a database. The system also includes at least one computing device for executing stored programmable instructions to: acquire data associated with the system component, determine which data from the received data are identified for extraction based at least in part on a tag, and extract the tagged data based on the determination. Learn More

A U.S. Patent Application for "Cells and Method of Cell Culture" was published by Pfizer on September 20th, 2018. The present invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods. Learn More

A U.S. Patent for a "Combination Stirrer" was awarded to Roche on September 18th, 2018. The present invention is a device comprising an agitator and a dialysis module within a cultivation vessel, wherein the agitator comprises: a shaft comprising a shaft axis; one anchor impeller comprising at least two opposing stirrer blades, wherein the stirrer blades of the anchor impeller have no pitch with respect to the shaft axis; and one or more inclined-blade stirrers each comprising at least two stirrer blades, wherein the stirrer blades of each inclined-blade stirrer have a pitch with respect to the shaft axis; wherein the stirrer blades of the anchor impeller are parallel to each other... Learn More

A U.S. Patent for an "Apparatus for Automated Determining of at Least Two Different Process Parameters" was awarded to Endress+Hauser on September 18th, 2018. The present invention is an apparatus for automated determining of at least two different process parameters of a process liquid of a process, especially a bioprocess, comprising: a first measuring cell, which is embodied to provide a first measurement signal dependent on a first process parameter of a first sample of the process liquid; a second measuring cell, which is embodied to provide a second measurement signal dependent on a second process parameter of a second sample... Learn More

A U.S. Patent Application for "Wireless Sensor Information Monitoring" was published by Lonza on September 13th, 2018. The present invention is a system, apparatus, and method for continuously monitoring data output from a wireless sensor on a mesh network at a frequency higher than mesh formation/propagation. For example, the system may include a wireless gateway, an amplifier coupled to the wireless gateway, a first antenna coupled to the amplifier, a second antenna coupled to the amplifier, and a wireless sensor. The wireless sensor may be a wireless temperature sensor configured to monitor the temperature of a cryopreserved material product. Learn More

A U.S. Patent for a "Liquid Cation Exchanger" was awarded to Evonik on September 11th, 2018. The present invention relates to a process for removing an organic compound having one or more positive charges from an aqueous solution, comprising the steps a) provision of the aqueous solution comprising the organic compound and of a hydrophobic organic solution which comprises a liquid cation exchanger, where the liquid cation exchanger is hydrophobic, and where the liquid cation exchanger has one or more negative charges and an overall negative charge, b) contacting the aqueous solution and the organic solution... Learn More

A U.S. Patent for "Sterile Affinity Chromatography Resin" was awarded to Genzyme on September 11th, 2018. The present invention provides methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chrom resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation... Learn More

A U.S. Patent Application for a "Method for Cell Line Development" was published by GE on September 6th, 2018. The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GOI genome target location and the introduction... Learn More

A U.S. Patent for "Methods, Surface Modified Plates and Compositions for Cell Attachment, Cultivation and Detachment" was awarded to Janssen Biotech on September 4th, 2018. The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells...
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A U.S. Patent for "Cell Culture Media and Methods" was awarded to Life Technologies on September 4th, 2018. The present invention describes compositions and methods for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release... Learn More

A U.S. Patent for "Methods of Producing Two Chain Proteins in Bacteria" was awarded to Genentech on September 4th, 2018. The present invention provides for methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes. Learn More

A U.S. Patent for "High pH Protein Refolding Methods" was awarded to Bristol-Myers Squibb on September 4th, 2018. The present invention is a method for refolding a denatured protein, comprising suspending a denatured protein in a suspension solution to obtain a composition comprising suspended denatured proteins; combining the composition comprising suspended denatured proteins with a solubilization buffer having a pH in the range of 11.5 to 12.8 to thereby obtain a composition comprising solubilized denatured proteins; and combining the composition comprising solubilized denatured proteins with a refold buffer having a pH in the range of 10 to 10.9. Learn More

A U.S. Patent for a "Regulated Vacuum Off-Gassing of Gas Filter for Fluid Processing System and Related Methods" was awarded to Life Technologies on August 28th, 2018. The present invention is a method for filtering a gas, the method comprising: delivering a gas into a compartment of a gas filter assembly, the compartment being at least partially bounded by a casing comprised of a flexible sheet of polymeric film; and applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly. Learn More

A U.S. Patent for a "Cell Culture Method in a Bioreactor System Using Micro-Carriers" was awarded to PBS Biotech on August 28th, 2018. The present invention is a system and method for compartmentalizing micro-carriers in a bioreactor includes a container configured to store micro-carriers and a vessel configured to contain a fluid so as to prevent the micro-carriers from entering the vessel during sterilization, shipping, storage, or other pre-use handling of the system. One or more addition lines connect the container and the vessel such that the vessel is in fluid communication with the vessel. Learn More

A U.S. Patent for "Disposable Bioreactors and Methods for Construction and Use Thereof" was awarded to Distek on August 28th, 2018. The present invention is a disposable bioreactor including a headplate and a stirrer. The headplate has at least one inlet port aperture and one outlet port aperture formed therein and is adapted to couple sealingly with a bag capable of receiving a culture medium. The stirrer is coupled to and extends from the headplate and is adapted to stir the culture medium when the headplate is coupled to the bag. The bioreactor is adapted to fit within the upper opening of the stand of an existing conventional glass bioreactor, so that the bag is suspended within the vessel.
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A U.S. Patent for "Mammalian Cell Culture Processes for Protein Production" was awarded to Bristol-Myers Squibb on August 28th, 2018. The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise the addition of glucocorticoid compound during the culturing period. The addition of glucocorticoid compound sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. Learn More

A U.S. Patent Application for a "Disposable Bioprocess System Supporting Biological Activity" was awarded to Stobbe Pharma Tech on August 23rd, 2018. The present invention is a Disposable Bioprocess System including a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. Learn More

A U.S. Patent for an "Audio Identification Device, Audio Identification Method and Audio Identification System"
was awarded to Sartorius Stedim on August 21st, 2018. The present invention is an audio identification device. The audio identification device comprises: at least one sensing device configured to capture an audio signal present in a working space of a processing setup including at least one processing device; and a processor configured to: receive information related to the at least one processing device; retrieve audio data stored in a database using the received information related to the at least one processing device; identify a state of the at least one processing device.
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A U.S. Patent for a "Particle Sensor with Interferent Discrimination" was awarded to BugLab on August 21st, 2018. The present invention provides methods and devices to measure particle suspension concentrations in the presence of potential interferents. Particle back-scatter readings are taken at light wavelengths that are absorbed by the medium before interacting with surrounding objects. Source-detector spacings are minimized compared to the mean absorbance path length of light, thereby maximizing the range of sensitivity to particle concentration. Discrimination against potentially interfering particles, such as bubbles, is provided... Learn More

A U.S. Patent Application for a "Virtual Transmitter for Bioreactor Automation System" was awarded to Finesse Solutions on August 16th, 2018. The present invention is a bioreactor monitoring and/or control system utilizing only non-dedicated user input and information display devices, a digital controller and software, said system comprising: i) at least one diagnostic probe for measuring operating conditions in a bioreactor; ii) signal conditioning and communication electronics which supply operating current and/or voltage to said probe and which convert the diagnostic signal into a format accessible. Learn More

A U.S. Patent Application for a "Cell Culture System and Method" was awarded to Lonza on August 16th, 2018. The present invention is a bioprocess system and a method for incubating, growing and harvesting cell cultures is described. Also disclosed is a bioprocess container that can be used with the system. In one aspect of the present disclosure, the bioprocess system includes bioprocess tubes and cell culture tubes having particular dimensions and being made from specific materials that allow the tubes to be welded together while preventing open connections and/or ruptures. Learn More